猕猴桃幼叶培养再生植株的细胞组织学研究

猕猴桃幼叶培养再生植株的细胞组织学研究 CYTOHISTOLOGICAL STUDIES OF PLANT REGENERATION FROM YOUNG LEAVES IN ACT1NIDIA MELANADRA 匡安秀 Kuang Anxiu and Li Rongqian 1 first-author 利容千 1 南京农学院 南京农学院 通过猕猴桃幼叶离体培养再生植株的结果表明:接种后3天,向着叶脉基部的切口处韧皮部、木质部和周围的薄壁细胞启动参与形成愈伤组织;培养18天后,不同培养基上分别分化出芽和根。愈伤组织长大,内部薄壁细胞分化成维管组织,周缘为分生细胞层,进一步分化成芽和叶原基;而生根培养基的愈伤组织产生类分生组织突破表皮,长出幼根,芽为外起源,根为内起源。 Through the culture of excised young leaves from Actinidia melanadra, the variation of cell and tissue structure in the process of the callus formation and organ differentiation was closely observed. The result indicted that after three days of incubation, the parenchymatous cells of phloem and xylem and those around vascular bundles at the cut surface of vein base were initiated in callus formation. By tenth day, the callus from the cut of vein base was visible, then the callus grew up and differentiated. The root primordia were originated endogenously, but shoot primordia exogenously. By 18th day after incubation, the root and the shoot were visible on two different medium. The initiation of callus from a piece of leaf was discussed. 1983-03-01 2021-04-01 3