水稻的未传粉子房培养

水稻的未传粉子房培养 CULTURE OF UNPOLLINATED OVARIES IN RICE 周嫦 first-author 杨弘远 田惠桥 刘中来 阎华 本文是四年来关于水稻未传粉子房培养的研究总结。内容包括三方面:影响培养的各种因素;雌核发育的胚胎学;再生植株的染色体与性状表现。对本项研究的结果作出了阶段性的结论。文末附培养技术流程以及本实验室有关未传粉子房培养方面的原始论文,以供参考。 This paper is a summary of four year’s work on in vitro culture of unpollinated ovaries in rice carried out in Department of Biology, Wuhan University. Following topics are included: Ⅰ Introduction. Ⅱ Factors affecting culture results: Cultivar response; Embryo sac stage; Cold treatment; Floral Parts; Liquid and solid media; Exogenous hormone in inducing medium; Concentration of sucrose in inducing medium; Differentiating medium; Rooting medium. Ⅲ Embryology of gynogenesis: Synergid apogamy; Egg cell anomalous division; Division of polar nuclei; Division of antipodal cells; Proembryo growth and callusing; Embryoid differentiation; Abnormal development of proembryo; Abortion of proembryo; Proliferation of somatic cells. Ⅳ Chromosome ploidy and characters of regenerated plants: Chromosome ploidy; Albinism; Other characters. Ⅴ Concluding remarks. Appendix: Technical procedures of rice ovary culture. References: A list of papers on ovary culture by the authors. 1983-04-01 2021-04-01 4