家蚕核型多角体病毒DNA的物化性质与感染活性

家蚕核型多角体病毒DNA的物化性质与感染活性 THE PHYSICAI CHEMICAI PROPERTIES AND INFECTIVITY OF NUCLEAR POLYHEDROSIS VIRUS OF BOMBYX MORI 马延高 Ma Yangao 1 2 first-author 陈蔚梅 Chen Weimei 1 2 王静怡 Wang Jingyi 1 2 燕小安 Yan Xiaoan and Ju Wenjun 1 2 卢文筠 1 2 黄可威 1 2 武汉大学病毒学系 武汉大学病毒学系 Dept, of Virology, Wuhan University) Huang Kewei Dept, of Virology, Wuhan University) Huang Kewei 中国农业科学院蚕业研究所 中国农业科学院蚕业研究所 从家蚕（苏 3 ×苏 4 ）核型多角体病毒（Nuclear Polyhedrosis Virus简称NPV）分离并提纯的DNA,具典型的核酸紫外吸收曲线,其超离心沉降系数(S 20·w )为40.36s.Tm值为84.8℃（于1×SSC中）,G+C含最为37.8%,热增色效应为30%,电镜下观察病毒DNA呈线状分子和开放环状分子,平均周长为36.26μ（分子量为75×10 6 道尔顿）。提纯的病病DNA具感染活性,当用DN ase 处理,丧失其感染力,而RN ase 处理则无多大影响。 Viral DNA was isolatcd from virus particles of nuclear polyhedrosis virus NPV of the silkworm, Bombyx mori （Chinese Su3 X Su4）and purlfied.The purified viral DNA had a typic ultraviolet absorption cutve of nucleic acid. The sedimentation coefficient (S 20 .w)of viral DNA determined in analytic centrifugaton was 40.36 S. The viral DNA gave a sharp melting curve. Its meiting temperature （Tm）（in l X SSC）is 84.8℃, and its thermal hyporchromic effect is 30%. The percentage of G+C of Bm NPV DNA was estimated from its Tm value to be about 37.82%. Electron microscopy of these DNA preparations revealed the linear and open circular molecules with contour Iength average 36.20μ（i. g. moleculer weight 75x10 6 daltons）. The purified viral DNA was infectious, whilc DNase - treated DNA lost its infectivity, RNase-treated DNA was unaffected. 1983-03-01 2021-04-01 3