猕猴桃幼叶培养再生植株的组织化学研究

猕猴桃幼叶培养再生植株的组织化学研究 CYTOHISTOLOGICAL STUDIES ON REGENERATION OF PLANT INDUCED FROM YOUNG LEAVES IN ACTINIDIA 匡安秀 Kuang Anhsiu and Li Rongqian 1 first-author 利容千 1 南京农学院 南京农学院 通过猕猴桃幼叶离体培养再生植株,观察了愈伤组织的形成和器官分化过程中淀粉粒的积累和RNA合成的动态。随着不同时期的生长和物质代谢的强弱,淀粉粒和RNA的增加或减少,淀粉粒往往出现在RNA之前,两者分布在组织中的部位恰好相反,淀粉粒多的部位,RNA很少,而RNA多的部位则无淀粉粒。此外还观察了愈伤组织形成与器官分化时,细胞分裂的昼夜功态变化与光照强度有关;细胞分裂的中期,昼夜间也有明显的高峰期。愈伤组织形成和芽的分化各个时期细胞代谢的慢弱与细胞分裂的频率高低基本相符。 Through the culture of excised young leaves from Actinidia the dynamic state of starch, RNA and cell division in the process of the callus formation and organ differentiation was closely observed. In the process of callus formation and organ differentiation, starch accu-mulation and RNA synthesis increased and decreased with the metabolic activi-ty. The starch appeared earlier than RNA, The distribution of starch and RNA was just opposite to each other. The rate of cell division was closly related with the matabolie activity at each stage of development. When cell division was most vigorous at the stage of callus formation, the change in the dymanic state at day and night is related with the light and darkness. Metaphase has an evident high peak. Finally, the relationship between the accumulation of starcb and callus formation and organ differentiation was discussed. 1984-02-01 2021-04-01 2