卡那霉素链霉菌中cAMP的存在及其与抗生素生物合成的关系*

卡那霉素链霉菌中cAMP的存在及其与抗生素生物合成的关系* RELATIONSHIP BETWEEN CYCLIC AMP AND ANTIBIOTIC BIOSYNTHESIS IN STREPTOMYCES KANAMYCETICUS 谭惠青 Tan Huiqing and Chen Shuyun first-author 陈漱涢 采用竞争性蛋白质结合放射分析法（competitive protein binding radioassay）对培养中的卡那霉索产生菌——卡那霉素链霉菌（Streptomyces kanamyceticus）胞内cAMP含量进行了测定。得到的结果是卡那霉素链霉菌在不同发酵时期均存在有胞内cAMP,其浓度变化于15—55pmol/mg菌丝干重之间。处于生长期（trophophase）的细胞胞内cAMP水平不断上升,达到高峰后,主生长期结束,进入抗生素合成期（idiophase）,合成期的细胞胞内cAMP水平急骤下降,并一直保持低水平。在培养基中加入外源cAMP,观察到卡那霉素合成明显减少。以上结果提示,cAMP与卡那霉素生物合成、与菌体的初级代谢转向次级代谢密切相关。 Estimating the levels of cyclic AMP by the competitive protein binding radioassay method, it was found that kanamycin producer, Streptomyces kanamyceticus, possess intracellular cyclic AMP through the fermentation course in broth medium. The level varies between 15—55 pmol/mg dry weight. In the trophophase cyclic AMP increased parallel to the growth. When the level of cyclic AMP achieved the peak, the main growth phase ended, then the level of cyclic AMP dccreased rapidly and maintained this low level throughout the idiophase. The biosynthesis of kanamycin was markedly inhibited by adding exogenous cyclic AMP. It was suggested that the variation of cyclic AMP levels in the cells of Streplomyces kanamyceticus were closely related to the shift from primary metabolism to secondary metabolism. 1984-01-01 2021-04-01 1