草地贪夜蛾多粒包埋核型多角体病毒DNA基因组的物理图谱及其部分DNA片段的克隆

草地贪夜蛾多粒包埋核型多角体病毒DNA基因组的物理图谱及其部分DNA片段的克隆 MOLECULAR CLONING AND PHYSICAL MAPS OF THE SPODOPTERA FRUGIPERDA NUCLEAR POLYHEDROSIS VIRUS DNA 严家骐 Yah Jiaqi first-author 通过使用交义双酶酶解草地贪夜蛾多粒包埋核型多角体病毒（Sf MNPV-D7）DNA所得片段的分析,以及将克隆的Sf MNPV-D7 DNA片段经 32 p标记后与该病毒的DNA片段杂交等方法,构建了有5种限制性内切酶（EcoRⅠ、Hind Ⅲ,Xho Ⅰ,BamH Ⅰ和Sac Ⅰ）限制位点的Sf MNPV-D7 DNA基因组的物理图谱。杂交资料表明,Sf MNPV-D7的基因组主要由独特的序列组成。用质粒PUC8作为载体,已获得Sf MNPV-D7基因组经EcoR Ⅰ和Hind Ⅲ酶解所得部分片段的克隆共25个。所得克隆的DNA中的一个片段,即EcoR Ⅰ-P,与苜蓿银纹夜蛾多粒包埋核型多角体病毒（Ac MNPV）的多角体蛋白基因同源,已用作Sf MNPV-D7基因组物理图谱的起点。 A physical map for the genome of Spodoptera frugiperda naclear poly-hedrosis virus （SfMNPV-D7） was constructed for the restriction endonucle-ases HindⅡ、EcoRⅠ, BamHⅠ,XhoⅠ and SacⅠ. It was derived by reciprocal double digests of isolated DNA fragments and hydridization of （32） P —labeled cloned viral fragments to unlabeled SfMNPV-D7 DNA restriction fragments. The hybridization data showed that the SfMNPV-D7 genome consists of mainly unique sequences. A partial library of SfMNPV-D7 EcoRⅠ and Hind Ⅲ fragments was produced using the plasmid vector PUC8. The cloned fragments were identified by restriction enzyme and hybridization analysis. One of the cloned fragments, EcoRI-P, was homologous to the Autographa californica nuclear polyhedrosis virus polyhedrin gene and was used as the origin of the SfMNPV-D7 restriction map. 1984-03-01 2021-04-01 3