蚕豆姐妹染色单体区分染色的孚尔根显示法

蚕豆姐妹染色单体区分染色的孚尔根显示法 AN IMPROVED METHOD FOR SISTER CHROMATID DIFFERENTIAL STAINING IN VICIA FABA CHROMOSOMES 余其兴 Yu Qixing first-author 本文报道蚕豆姐妹染色单体分染（SCD）的一种改良技术,即BrdU—Feulgen法。种子萌发后,剪取主根根尖,用清水培养,当大多数侧根长0.5—1.5厘米时,放入BrdU(5×10 -4 M)溶液中培养两个细胞周期,随后用秋水仙素预处理,甲醇—冰醋酸固定,再在37℃的3.5NHC1中延长水解90—110分钟,以Schiff试剂染色,压片。此法具有简便、快速和分染效果较好的特点。其分染结果是与FPG法以及BrdU—Giemsa法相反的B—浓染型。本文还探讨了蚕豆细胞短周期问题,以及蚕豆SCE作为诱变因素检测手段的实际应用价值。 An improved BrdU-Feulgenmethod for SCD in Vicia faba chromosomes has been developed. The primary roots of the seeds germinated in, wet sawdust were decapitated anb suspended in clear water to promote the growth of their lateral roots. When most of the lateral roots were grown up to be about 0.5-1.5 cm in length,exposed them to an aqueous solution of 500μM BrdU in the dark at 25℃ for two consecutive rounds of replication Subsequently, their tips were cut and treated with colchicine solution（0.05%）for four hours and fixed in 1:3 acetic-alcohol for two hours Then, these root-tips were hydrolyz-ed in 3.5N HC1 at 37℃ fot 90-110min, and stained with Schiff’s reagent for 30min. or more, and eventually squashed in 45% acetic-acid. This method gave a satisfactory differential staining of sister chromatids and was proved to be a rather rapid and simple procedure. The effect of diff-erential staining in this procedure is contrary to that of the conventional FPG and BrdU-Giemsa techniques. This improved method might be an effective means for biological monitoring of environmental pollution, Some problems concerned are discussed. 1984-03-01 2021-04-01 3