水溶性蛋白质在鸡胚发育中变化的电泳分析

水溶性蛋白质在鸡胚发育中变化的电泳分析 ELECTROPHORETIC ANALYSIS OF THE CHANGES OF WATERSOLUBLE PROTEINS IN THE DEVELOPING cHICK 刘玮 first-author 何海平 吴熙载 用PAGE及凝胶扫描技术检测了鸡胚发育中卵白、蛋白羊水和卵黄内水溶性蛋白质部分的变化。也测定了各期羊水蛋白质浓度的变化。蛋白羊水各种组份基本上来自卵白,但两者各组份的相对浓度有差异,蛋白质浓度在14D达极大值,以后又趋下降。1—7D时期,卵黄中的水溶性蛋白质PAGE不出现明显的新电泳区带,主要地有水份进入卵黄基质。8D至胚胎发育完成的期间,PAGE区带变化很大。卵清蛋白等卵白组份在发育较晚阶段——约14D左右,进入到卵黄基质中。 Changes of the watersolable protein fractions in egg—white, amniotic fluid and yolk of the developing chick have been examined by PAGE and gelscanning techniques. The protein concentration of amniotic fluid in different periods has also been made. The compositions of the protein in amnion fluid originate largely from egg-white, but there are differences on relative concentration for each constituent. On the 14th day, the protein concentration of amniotic fluid reaches maximum and then it turn to decrease. During 1—7 day, the PAGE of the watersoluble proteins in the yolk shows no new distinct electrophoretic zones. Mainly, the moisture content might inter the yolk matrix. There are conspicuous changes in the PAGE zones during the period from the 8th day to the end of embryogenesis. There are some ovalbumin etc. egg—white fractions introduced into the yolk matrix in the later developmental Phase—around the 14th day. 1984-04-01 2021-04-01 4