柠檬醛抑制黄曲霉生长相关机理的研究

柠檬醛抑制黄曲霉生长相关机理的研究 Study of the Relative Mechanism for Citral Antigrowth to Aspergillus flavus 罗曼 LUO Man 1 1 2 3 first-author 邹国林 2 1 2 3 蒋立科 ZOU Guo-lin 1 1 2 3 武汉大学生命科学学院 武汉大学生命科学学院 1 College of Life Sciences, Wuhan University 1 College of Life Sciences, Wuhan University 安徽农业大学生物工程系,湖北,武汉,430072 安徽农业大学生物工程系,湖北,武汉,430072 2 Department of Biological Engineering, Anhui Agricultural University, Heifei 2 Department of Biological Engineering, Anhui Agricultural University, Heifei ,湖北,武汉,430072 ,湖北,武汉,430072 对经柠檬醛损伤的黄曲霉 ,采用 Irwin等人方法测定质膜通透性 ,Miller方法测定试验菌对还原糖和蛋白质吸收利用率 ,Hodge等人方法纯化质膜 [Na+,K+]- ATPase,并用 L iberg方法测定其活性 ;以琥珀酸、丙酮酸和 α-酮戊二酸为底物测定 Mt呼吸速率 ,并结合扫描电镜观察和彗星图像分析 ,发现该醛能破坏细胞壁、质膜、Mt和膜选择通透性 ,并损伤核 DNA,而引起细胞的死亡 ,揭示这种单体芳香醛抗霉是多途径的复合作用 ,为其走向粮油、食品贮藏领域提供了理论依据 . A. flavus was inhibited and its spore didn’t germinate when inoculated in citral culture medium. By Irwin’s et al. method, the selective permeability of plasma membrane of hyphabody was measured; by Miller’s method, the rate of absorbing reducing sugar and protein was measured;by Hodge’s method [Na +,K +]-ATPase was purified, and its activity was measured by Liberg’s method;when the succinate, pyruvic acid and α-ketoglutaric acid respectively used as substrates, the Mt’s respiration rate was measured;by scanning electron microscope （SEM）, the spore andthe Mt were observed;by the analysis of SCGE (single cell gel electrophotesi,image,the damaged DNA was observed and analysed;finally the process was found that the aldehyde could damage the cell wall, plasma membrane, Mt, the selective permeability of plasma membrane and nucleic DNA, which brought on the process of death. The result suggested that citral resistance growth to A.flavus was a kind of compound function in many ways. This provided theoretical basis for the use of citral in preventing grain oil and food from mildewing. 柠檬醛 黄曲霉 抑制生长 相关机理 Citral Aspergillus flavus Inhibition growth Relative mechanism Q946.827 国家档案局资助项目 （ 90 3-保 -0 2 ） 2001-06-01 2021-04-01 6