胱氨酸在铜（Ⅱ）—氨—氯化铵溶液中的极谱吸附波

胱氨酸在铜（Ⅱ）—氨—氯化铵溶液中的极谱吸附波 POLAROGRAPHIC ADSORPTIVE WAVE OF CYSTINE IN Cu 2+ —NH 3 —NH 4 Cl MEDIUM 吴志强 Wu Zhiqiang and Zhao Zaofan first-author 赵藻藩 < 正 > 胱氨酸、半胱氨酸在汞电极上的极谱行为研究较少。Kolthoff等用直流极谱法研究了Cu 2+ —NH 3 —NH 4 Cl溶液中胱氨酸、半胱氨酸的极谱行为,得到了铜络合物的扩散波。U·Forsman用脉冲阴极溶出伏安法研究了胱氨酸及半胱氨酸与铜的络合物的极谱行为。我们用导数示波极谱法,发现在Cu 2+ —NH 3 —NH 4 Cl溶液中,当有微量胱氨酸存在时,在-0.76伏处能产生一个灵敏的极谱吸附波,半胱氨酸在同样的条件下也可产生相同的极谱波,利用此极谱波可测定微量胱氨酸或半胱氨酸,检测下限为1×10 -7 M（胱氨酸）及 In ammonia buffer sotution （pH=9.3）, in the presence of definite amounts of Cu 2+ , cystine（RSSR） or eysteine（RSH） can produce a sensitive adsorptive wave with a sharp peak shape on derivative single sweep polarography, E P =-0.76V（vs. SCE）. The detection limits are 1×10 -7 M（RSSR） and 2×10 -7 M （RSH）. There is alinear dependence or peak height on concentration over a range of 2×10 -7 -1×10 -5 M（RSSR） or 4×10 -7 -2×10 -5 M（RSH）. Small amounts of other sulfur containing aminoacids and many common ions do not interfere the determination. Good results were obtained in determining the total amounts of RSSR and RSH in hair samples. 1985-04-01 2021-04-01 4