大直径单层混合脂质体的制备及其在膜融合研究中的应用

大直径单层混合脂质体的制备及其在膜融合研究中的应用 PREPARATION AND SEPARATION OF DMPC—DMPA MIXED LARGE UNILAMELLAR LIPOSOMES AND IT’S APPLICATION TO MEMBRANE FUSION STUDY 彭银祥 Peng Yinxiang 1 2 first-author E. V. Orsi 1 2 M. Petersheim 1 2 武汉大学 武汉大学 Wuhan University) E. V. Orsi, M. Petersheim Wuhan University) E. V. Orsi, M. Petersheim 美国西东大学 美国西东大学 本文描述一种制备大直径单层均一的DMPC—DMPA混合脂质体的方法;由该法制得的脂质体分A组和B组,A组载入水溶胜荧光物质Tb（Citrate） 3 , B组载入水溶性物质DPA,采用Wilshut[1]等提出的荧光方法研究Ca 2+ 诱导下此混合脂质体A和B的融合程度和融合速度以及相应的Ca 2+ 浓度,探索Ca 2+ 促脂质体的融合作用和凝集作用的情况。 A method on preparation and Separation of DMPC—DMPA mixed large unilamellar liposomes was described. The liposomes obtained can be examined by Scanning electron microscope and the diameter of liposomes was large than lμ. This kind of liposome was Suitable for membrane fusion study as cell model. Furthermore, analysis on fluorescent strength shows that Ca 2+ -indused membrane fusion not only depends on the size and concentration of liposome but also on the concentration of Ca 2+ . The fusion and aggregation of membranes can be generated at the same time. 1986-03-01 2021-04-01 3