分月扇舟蛾颗粒体病毒DNA的某些理化性质的分析

分月扇舟蛾颗粒体病毒DNA的某些理化性质的分析 CHARACTERIZATION OF CLOSTERA ANASTOMOSIS GV-DNA 孟小林 Meng Xiaolin and Liu Niencui first-author 刘年翠 分月扇舟蛾（Clostera anastomosis 简称Cas）颗粒体病毒（Granulosis Virus简称GV）的核酸与二苯胺呈天蓝色阳性反应,从其核酸在0.1×SSC溶液中热变性紫外吸收曲线来看,具有28.3%的热变性紫外吸收增色效应,这都表明CasGV的核酸类型为双链DNA,其Tm值为72.4℃,G+C含量为45%,用限制性内切酶EcoR—Ⅰ酶切CasGV—DNA,琼脂糖凝胶电泳呈现出22个DNA片段带谱,积加得其分子量为90×10 道 尔顿。 Granulosis Virus of Clostera anastomosis gave positive diphenylamine reaction and its thermal hyperchromic effect was 28.3%. The results showedthat nuclear acid of CasGV was dsDNA. Its melting temperature（Tm） in 0.1 ×SSC was 72.4℃ and the guanine—plus—cytosine content determined by Tm was45%. The Viral genome was examined with the restriction endonuclease EcoR—I, agarose—gel electrophoresis of the digested DNA yielded 22 bands, and a total molecular weight is 90×10 6 d 1986-03-01 2021-04-01 3