N-取代二苯并氮杂冠醚的动态改性高效液相色谱

N-取代二苯并氮杂冠醚的动态改性高效液相色谱 HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH DYNAMICALLY MODIFIED SILICA FOR N-SUBSTITUTED DIBENZO-AZACROWN ETHERS 达世禄 first-author 赵文宽 刘丽君 盛平 吴成泰 动态改性色谱是近几年来高效液相色谱的新进展。本文分别选择季铵盐,非离子型表面活性剂、聚乙二醇等作改性剂,研究亲脂性的N—取代二苯并氮杂冠醚在动态改性色谱中的保留机理和影响保留的因素。在无改性剂存在下, 甲醇一水,甲醇作流动相,不能从硅胶柱上将冠醚洗脱。用高甲醇含量的流动相,动态改性硅胶色谱可有效分离冠醚化合物,其分离选择性较优于键合ODS柱。 Chromatography with dynamically modified silica is the new development in HPLC in recent years. In this paper, the retention mechanism of N-substituted dibenzo-azacrown ethers in HPLC with dynamically modified silica and the influence of various factors on retention have been investigated by use of quatenary ammounium compounds, non-ionic surfactant, polyetliylene glycol as modifiers. Under the condition to use methanol or methanol-water without modifiers as mobile phaes, the crown ethers can not be eluted from silica. Using methaolhigh containing mobile phase, the crown ethers have been separeted effectively by HPLC with dynamically modified silica, its selection for separation is better than bonded phase ODS. 中国科学院科学基金 1986-04-01 2021-04-01 4