小鼠乳腺癌细胞系MA782/5S-8102染色体Giemsa分带核型研究

小鼠乳腺癌细胞系MA782/5S-8102染色体Giemsa分带核型研究 STUDY OF GIEMSA-BANDING KARYOTYPE OF MOUSE MAMMARY ADENOCARCINOMA CELL LINE（MA782/5S-8102） 黄佩霞 first-author 李文鑫 胡解郁 对小鼠乳腺癌细咆系MA782/5S-81002进行了Giemsa分带的遗传学分析。结果表明,MA782/5S-8102细胞系是一个亚四倍体细胞系,染色体众数范围为61—68,主流数为65.有8种出现频率较高的标记染色体,即16/15,16/4,19/4,19/5,19/8和15/13罗伯逊易位的近中或近端着丝点染色体,分别称为M 1 ,M 2 ,M 3 ,M 4 ,M 5 ,M 6 ,另外还有一类微小染色体（M 7 ）,包含三种情况,大微小染色体,小微小染色体和点状染色体。 2号染色体长臂末端缺失是MA782/5S-8102细胞系突出特征。除此以外,还存在着其它的畸变类型。我们认为MA782/5S-8102细胞染色体这些多样型畸变显示出了它的恶性特征。本文对染色体畸变和癌变关系进行了讨论。同时也对染色体畸变与癌基因的关系进行了探讨。 The mouse mammary adenocarcinoma cell line （MA782/5S-8102） was analysed cytogenetically with technique of G-banding. The results are indicated that（MA782/5S-8102）is a cell line of subtetraploid having a modal number of 61-68 with more 8 makers chromosomes which are 19/4, 16/15, 15/13, 16/4, 19/8 and 15/10 submetacentric chromosomes and subtelocentric chromosomes of Robertsonian translocation, called M 1 , M 2 , M 3 , M 4 , M 5 and M 6 . The others are minichromosomes and a terminal deletion of the long are in chromosome, No, 2 that is a specific feature of cellular line MA782/5S-8102. The relationship between chromosomal aberration and tumorigenesis is discussed. The chromosomal aberration asscciated with oncogen is also. 1986-04-01 2021-04-01 4