用固定化酶反应的热化学监测法测定糖类

用固定化酶反应的热化学监测法测定糖类 DETERMINATION SUGARS BY THERMOCHEMICAL MONITORING OF IMMOBILIZED ENZYME REACTONS Richard K.Owusu 1 2 3 4 5 6 first-author Arthur Finch 1 2 3 4 5 6 Department of Chenlistry Royal Holloway and Bedford Colleges Department of Chenlistry Royal Holloway and Bedford Colleges UniveYsity of Londoil UniveYsity of Londoil Egham Egham Surrey Surrey TW20 0EX TW20 0EX ,UK ,UK 本文介绍了由固定在多孔玻璃载体上的葡萄糖氧化酶以及过氧化氢酶或己糖酶所催化的反应籍热化学监测法对葡萄糖,果糖、甘露糖及蔗糖进行测定。用这种方法可检测的糖的最小量可达7～25×10 -8 摩尔,精度约为±4%,仅取决于所使用的酶和被检测的糖类。 The determination of glucose, fructose, mannose and sucrose using thermochemical monitoring of reactions catalysed by the enzymes glucose oxidase and catalase or hexokinase, immobilized on porous glass, is described. As little as 7—25×10 -8 mole of sugar could be determined, depending on the enzyme used and the sugar determined, with a precision of about ±4%. 1986-01-01 2021-04-01 1