缺刻转移和DNA杂交组建枯草杆菌噬菌体DNA的物理图谱

缺刻转移和DNA杂交组建枯草杆菌噬菌体DNA的物理图谱 NICK TRANSLATION AND DNA HYBRIDIZATION FOR CONSTRUCTION OF A PHYSICAL MAP OF BACILLUS SUBTILIS PHAGE φ105 DNA 齐义鹏 Qi Yipeng 1 2 first-author J.Lampel 1 2 武汉大学病毒学系 武汉大学病毒学系 Dept. of Virology, Wuhan University) J. Lampel Dept. of Virology, Wuhan University) J. Lampel 美国俄亥俄州立大学微生物系 美国俄亥俄州立大学微生物系 用缺刻转移法制备放射性探针DNA,以脱水的琼脂糖凝胶作为杂交的支持物,进行DNA-DNA的分子杂交,组建了枯草杆菌噬菌体105φDNA的EcoR1和Hpa1片段的物理图谱。EcoR1片段的顺序是DGIEBHFC;Hpa1片段的顺序是DICHGAFEJ.本文对组建物理图谱的几种方法的优缺点进行了讨论。 Using nick translation for radioactive probe DNA and dehydration gel instead of Southern blot for hybridization matrix, a map of Bacillus subtilis phage φ105 DNA for EcoRl and Hpal fragments was coasted. The order of EcoR1 fragments is DGIEBHFC, and that of Hpal Jraments is DICHGAFEJ. Discussion of advantages anddefects for partial digestion and hybridization has been done in the present paper. 1986-01-01 2021-04-01 1