酶解直读法测定番茄叶绿体4.5S rRNA的部分序列

酶解直读法测定番茄叶绿体4.5S rRNA的部分序列 THE DETERMINATION OF PARTIAL NUCLEOTIDE SEQUENCE OF 4.5SrRNA FROM TOMATO CHLOROPLASTS BY ENZYMIC METHOD 肖啸 Xiao Xiao 1 2 first-author 谷丽雅 Gu Liya and Wang Esheng 1 2 王鄂生 1 2 程振起 1 2 赵晜 1 2 武汉大学生物系 武汉大学生物系 Depa tment of Biology, Wuhan University) Cheng Zhenqi and Zhao Kun Depa tment of Biology, Wuhan University) Cheng Zhenqi and Zhao Kun 中国科学院生物物理所 中国科学院生物物理所 从番茄（Lycopericun esculentnm Mill）叶中分离纯化了叶绿体4.5S rRNA。用酶解直读法分别测定其5′末端的部分核苷酸序列为5′-OH GAAGGUCACGCGAGACGACCCUUUAUCUUACGAUAGUGUC……3′末端部分核苷酸序列为……UGGGCAUCCUACAGAUCGGGUGACUUGAAC OH .-3′以上序列与已知烟草叶4.5SrRNA序列的相应部分具有很大的同源性。文中还讨论了其生物学意义。 The chloroplast 4.5S rRNA was isolated and purified from the leaves of tomato （Lycopersicun esculentum Mill.）The 5′ terminal and 3′ terminal nucleotide sequences were determined by enzymic sequencing method respectively to be: 5′-OH GAAGGUCACGGCGAGACGAGCCGUUUAUCAUUACGAUA GGUGUC…………………UGAGGCAUCCUUAACAACAGAUCGGGUAGACUUGAAC OH -3′ These sequences shown strong homology with that of tobacco（Nioctiana tabacum L.）. The biological significanee of which is discussed. 1986-01-01 2021-04-01 1