棒状细菌(棒杆菌、短杆菌)的原生质体形成、再生及融合

棒状细菌(棒杆菌、短杆菌)的原生质体形成、再生及融合 STUDIES ON PROTOPLAST FORMATION, REGENERATION AND FUSION OF CORYNEFORM BACTERIA 高忠翔 Kao Zhongxiang and Chen Shuyun 1 first-author 陈漱涢 1 清华大学生物科学与技术系 清华大学生物科学与技术系 采用甘氨酸-青霉素复合预处理-蛋清溶菌酶酶解的方法制备原生质体,形成率可达80—90%。在对数中期加入青霉素预处理,形成率最高。EDTA和青霉素预处理及青霉素加入时的菌体生理状态都对原生质体再生有较大的影响。采用软琼脂上层再生培养基铺层法和在酶液及原生质体稀释液中加入牛血清白蛋白的方法都可使再生率提高。用间接选择法获得了原生质体种内融合子,融合频率为6.8×10 -7 。 Protoplasts were prepared with giycine-penicillin pretreatment and lysozyme treatment. The formation frequency of protoplasts was highest when penicillin was added in midexponential growth phase. The regeneration of protoplasts was affected by EDTA, penicillin preteatment and the physiological state of the bacteria, Both the soft agar method and the addition of Bovin Serum Album in the lysozyme solution and 1he dilution fluid can enhance the frequency Of regeneration. The intraspecific fusants were obtained with indirect selective method, The fusion freqnency was 6.8×10 -7 of plated protoplasts. 原生质体形成与再生 原生质体融合 棒状细菌 Protoplast formation and regeneration Protoptast fusion Coryneform bacteria 中国科学院科学基金 1987-01-01 2021-04-01 1