慢性粒细胞白血病急变与bcr/abl,p53,p16基因相关性研究

慢性粒细胞白血病急变与bcr/abl,p53,p16基因相关性研究 The Study on bcr/abl,p 53 ,p 16 Gene in the Evolution of CML to Blast Crisis 李慧玉 LI Hui\|yu 1 1 2 3 first-author 邹国林 2 1 2 3 邹平 ZOU Guo\|Lin 1 1 2 3 武汉大学生命科学学院!湖北武汉430072华中科技大学同济医学院协和医院 武汉大学生命科学学院!湖北武汉430072华中科技大学同济医学院协和医院 College of Life Sciences, Wuhan University Wuhan College of Life Sciences, Wuhan University Wuhan ,湖北,武汉,430022 ,湖北,武汉,430022 Union Hospital,Tongji Medical College,Huazhong University of Science and Technology Union Hospital,Tongji Medical College,Huazhong University of Science and Technology 华中科技大学同济医学院协和医院,湖北,武汉,430022 华中科技大学同济医学院协和医院,湖北,武汉,430022 应用 RT- PCR、PCR- SSCP方法对 33例慢性粒细胞白血病 （Chronic myeloid leukem ia,CML）进行了bcr/ abl,p5 3,p16基因检测 .发现 33例 CML 中 32例 bcr/ abl基因阳性 （97.0 % ） ,2 2例慢性期无 p5 3基因突变 ,而11例急变组中一例外显子 5和一例外显子 7发生 p5 3基因突变 ,且均发生于急粒变组 ,2例出现 p16基因纯合缺失 ,且均发生在急淋变组 ,33例 CML 均未见 p16基因突变 .结果提示 :bcr/ abl基因与 CML 发生有关 ,而 p5 3基因突变可能与 CML 急粒变有关 ,p16纯合缺失可能与 CML 急淋变有关 bcr/abl,p53,p16 gene were detected by means of RT\|PCR,PCR\|SSCP methods,respectively.We found that 32 out of 33 cases with CML were bcr/abl gene positive （97%）.The mutation of p53 was found in none of them in chronic phase,while in 18%（2/11） of them in myeloid crisis and 2 homozyous deletion of p16 gene in lymphoid blast crisis,It suggested that there was intimate relationship between homozynous deletion of p16 gene and CML in lympoid blast crisis、between p53 mutation and CML in myeloid blast crisis. bcr/abl基因 p53基因 p16基因 慢性粒细胞白血病 bcr/abl gene p53 gene p16 gene chronic myeloid leukemia R733.7 2001-02-01 2021-04-01 2