不同细胞质雄性不育小麦中依赖钙/钙调素的蛋白激酶的SDS-PAGE电泳图谱分析

不同细胞质雄性不育小麦中依赖钙/钙调素的蛋白激酶的SDS-PAGE电泳图谱分析 The SDS-PAGE of Ca 2+ /CaM-dependent Protein Kinase in Different Types of Cytoplasmic Male Sterile Wheat 史红梅 SHI Hong\|mei\+1 1 2 first-author 何之常 HE Zhi\|chang\+2 1 2 胡德文 HU De\|wen\+2 1 2 徐乃瑜 XU Nai\|yu\+2 1 2 华中农业大学理学系,湖北,武汉,430071 华中农业大学理学系,湖北,武汉,430071 Department of Sciences, Huazhong Agriculture University Department of Sciences, Huazhong Agriculture University 武汉大学生命科学学院,湖北,武汉,430072 武汉大学生命科学学院,湖北,武汉,430072 College of Life Sciences, Wuhan University, Wuhan 430072,Hubei,China) \ \ College of Life Sciences, Wuhan University, Wuhan 430072,Hubei,China) \ \ 以几种新型细胞质雄性不育型小麦为材料 ,对分蘖期小麦叶片中的依赖钙 /钙调素的蛋白激酶 （Ca2 + /Ca M- PK）的 SDS- PAGE电泳图谱进行了分析 ,实验结果表明 ,不育系与保持系的蛋白激酶酶带之间存在差别 ,并且不育系之间也有差别 ,说明不育细胞质对核基因的表达也有影响 ,这可能与雄性不育有关 ,而且不育材料中存在潜在优势 ,可用来选育具有更强优势组合的核质杂种 Using four types（C,G,M,Mt 2） of cytoplasmic male sterile wheat as experiment materials, their maintainer Chinese spring wheat （T.aestivum） as contrast, the zymograms of Ca 2+ /CaM-dependent protein kinase were studied. The electrophoresis were carried out after crude enzyme of Ca 2+ /CaM-PK were extracted from leaves in tillering stage. The bands were well separated upon linear gradient SDS-PAGE（6%\|15%）.There existed difference among male sterile materials, maintainer and Huamai No.8. So the cytoplasm have different effect on the expression of gene, which probably associated with the cause of male sterility.The new male sterile wheat may be used to create new hybrid. 依赖钙/钙调素的蛋白激酶 雄性不育 杂种优势 小麦 Ca2+ /CaM-dependent protein kinase male sterility hybrid heterosis wheat Q945;S512 国家基础性研究攀登计划资助项目! （PD-XZ- -4 b） 2001-02-01 2021-04-01 2