水稻间期核、粗线期和有丝分裂中期染色体FISH分辨率的比较

水稻间期核、粗线期和有丝分裂中期染色体FISH分辨率的比较 Comparative Study of FISH Resolution on Interphase,Pachytene and Metaphase Chromosomes in Rice 李宗芸 1 2 first-author 熊志勇 1 2 金危危 1 2 宋运淳 1 2 武汉大学植物发育生物学教育部重点实验室,武汉大学植物发育生物学教育部重点实验室,武汉大学植物发育生物学教育部重点实验室,武汉大学植物发育生物学教育部重点实验室,湖北,武汉,430072 武汉大学植物发育生物学教育部重点实验室,武汉大学植物发育生物学教育部重点实验室,武汉大学植物发育生物学教育部重点实验室,武汉大学植物发育生物学教育部重点实验室,湖北,武汉,430072 徐州师范大学生物系,江苏徐州221009,湖北武汉430072,湖北武汉430072,湖北,武汉,430072 徐州师范大学生物系,江苏徐州221009,湖北武汉430072,湖北武汉430072,湖北,武汉,430072 利用水稻第 4染色体上的两个BAC（bacterialartificialchromosome）克隆作为探针 ,对水稻间期核、减数分裂染色体和有丝分裂中期染色体进行了荧光原位杂交 （fluorescenceinsituhybridization ,FISH） 结果表明 ,它们的杂交信号在水稻第 4染色体短臂上 ,有丝分裂中期染色体上两信号完全重叠 ,而在早粗线期染色体上可以分开 已知它们间的遗传距离为 2cM ,在粗线期染色体上两信号点彼此邻近但未重叠 ,两信号点中心的物理距离为 0 .36μm ,在间期核上两个信号点完全分开 ,相距 （2 .0 6± 1.15 ） μm 说明在水稻第 4染色体上 ,减数分裂粗线期FISH可以分辨相距约 2cM的两个标记 讨论了水稻减数分裂粗线期染色体FISH的技术及其优越性 Two BAC （bacterial artificial chromosome） clones on chromosome 4 of rice were hybridized to interphase nuclei, meiotic chromosomes and metaphase chromosomes of rice. The results showed that the signals of BAC clones H0102C09 and H0201G08 were located on the short arm of chromosome 4, overlapped completely on metaphase chromosomes, but separated on propachytene chromosomes. The genetic distance between them is 2 cM. Two signals derived from the tested BAC clones were connected to each other but not completely overlapped on pachytene chromosomes, and the central distance of the two signal spots was about 0.36 μm, and separated completely on interphase nuclei, and the physical distance was （2.06±1.15） μm. These demonstrated that the resolution of pachytene\|FISH of chromosome 4 was about 2 cM in rice. The technique and advantage of meiotic FISH in rice were discussed. 荧光原位杂交技术 分辨率 粗线期染色体 水稻 fluorescent in situ hybridization （FISH） resolution pachytene chromosomes rice S511 国家自然科学基金 （3 990 0 0 83 ）;教育部高等学校博士学科点专项科研基金 （970 4860 7）资助项目 2002-04-01 2021-04-01 4