澳洲黑鸡胚胎发育过程中卵黄液晶化与酶关系的研究

澳洲黑鸡胚胎发育过程中卵黄液晶化与酶关系的研究 STUDIES ON THE RELATIONSHIP BETWEEN LIQUID CRYSTAL FORMATION AND ENZYME ACTION IN THE YOLK DURING DEVELOPMENT OF THE AUSTRALORP EMBRYO 杨力 Yang Li 1 first-author 中国医学科学院基础医学研究所 中国医学科学院基础医学研究所 用偏光显微镜、酶组织化学、电镜细胞化学、同工酶电泳等方法研究了澳洲黑鸡胚发育过程中卵黄液晶化与酸性磷酸酶（ACPase）和非特异性酯酶的关系。结果表明酶参与卵黄液晶化过程。此外,还探讨了卵黄液晶化的意义。 It was found that the yolk droplets must undergo phase transition before being digested and absorbed, showing a marked change of state from non—ordered isotropic to ordered anisotropic figures. But so far it has not been known clearly whence the formation of liquid crystals begins to take place within the organisms, or what is the limiting factor which makes possible such formation. The present paper reports the author’s observational and experimental results on the study of the relationship between liquid crystal formation and enzyme action in the yolk during development of the Australorp embryo. In order to carry on the research work correctly, the following techniques have been adopted: 1. Polarizing microscopy, 2. Enzymatic histochemistry, 3. EM cytochemistry, 4. Isoenzyme electrophoresis, From the data obtained we are able to come to the conclution as follows: 1. Enzyme action（for example ACPase artd non-specific esterase in our experiment）is at least one of the important factors causing the formation of liquid crystals. 2. The biological significance of liquid crystals remains worthy to be further studied. It is now believed that yolk material must first of all turn to be liquid crystals and then enter the septa of the yolk sac in an active state, being converted into simpler substances and absorbed by the vascular system in order to feed the developing embryo. Therefore the liquid crystal formation in the yolk represents an intermediate stage of the yolk digestion. 卵黄 液晶化 ACPase 非特异性酯酶 1987-03-01 2021-04-01 3