c-myc和c-ras基因在正常、化学转化和肿瘤细胞中的表达

c-myc和c-ras基因在正常、化学转化和肿瘤细胞中的表达 EXPRESSION OF C-MYC AND C-RAS ONCOGENES IN NORMAL、CHEMICALLY TRANFORMED AND TUMOR CELLS 李文鑫 Li wenxin first-author 以地鼠胚成纤维细胞（HEF）为对照,在BA10-IRS-/M - 和RA-T细胞中,观察了c-myc和c-ras基因的表达。同时,也观察了这些细胞在幼地鼠中的致瘤率。结果指出,在这三种细胞中c-ras基因均处于活化表达状态。而c-myc基则不同,在HEF细胞中未发现表达;但是在BA-T细胞中c-myc的表达水平比在BA10-IRS - /M - 细胞中高10倍以上。另外,BA10-IRS - /M - 细胞的致瘤率为100%,而未发现HEE细胞的致瘤现象。我们认为,在这三种不同状态的地鼠细胞中c-ars基因的表达可能与细胞正常生长代谢有关。c-myc基因的表达或是c-myc基因与c-ras基因的协同作用与细胞的恶性状态和肿瘤的发生有关。 Cellular oncogenes are conserved with great fidelity across a broad span of evalution. This avid conservation suggests possible roles in critical physiologic functions. In this study, we examined the expression of c-ras and c-myc oncogenes in normal cells（FEH）, chemically transformed cells（BA10-IR S - /M - ）and tumor cells（BA-T）. The results showed that c-ras oncogene was expressed in these three different cells A level of c-ras expression from FEH cells was similar to that found in BA10-IRS - /M - and BA-T cells. However, c-myc oncogene was expressed in BA10-IRS - /S - and BA-T cells, and is not present in FEH cells The level of c-myc expression in BA-T cells was about 10 fold higher than in BA10-IRS - /M - cells In addition, we determined tumor producing capacity of FEH and BA10—IRS - /M - cells in newborn hamsters. The BA10-IRS - /M - cells（10 4 ）and FEH （10 5 ）were individually inoculated in 5 newborn hamsters The tumor producing capacity of BA10-IRS - /M - cells is a 100%, but FEH is not These results indicate that expression of the c-ras oncogene would be related to the normal growth of cells, and that cooperation of the c-ras with the c-myc would play an important role in transformation of cells and the formation of tumors. 表达 致癌基因c-ras/c-myc 肿瘤发生力 expression oncogenes c-ras/c-myc tumor producing capacity 1987-02-01 2021-04-01 2