两种不同的染色方法比较分析茶毛虫核型多角体病毒结构多肽

两种不同的染色方法比较分析茶毛虫核型多角体病毒结构多肽 COMPARISION OF STRUCTURE POLYPETIDES FROM EUPROCTIS PSEUDOCONSPERSA NPV IN SDS-PAGE BY TWO DIFFERENT STAINING METHODS OF AMMINO-SILVER AND COOMASSlE BRILLIANT BLUE 孟小林 first-author 刘年翠 采用考马斯亮蓝染色检测了丙烯酰胺凝胶中的茶毛虫核型多角病毒（Euproctispseudoconspersa Nuclear Polyhedrosis Virus简称EpNPV）的结构多肽,结果表明,EpNPV的包含体蛋白仅为一条主带,其分子量为27000d,病毒粒子结构多肽有17条。采用铵银染色法检测EpNPV的包含体蛋白要比考马斯亮蓝染色法灵敏50倍,比考马斯亮蓝染色检测EpNPV病毒粒子结构多肽灵敏8倍。铵银染色法的结果表明,EpNPV的病毒粒子结构多肽有25种,比考马斯亮蓝染色法多显示出了8条带,这25条结构多肽的分子量范围在8—115×10 3 d。由此可见铵银染色法具有灵敏度高,反差强以及简便快速等优点。 Strcture polypetides of nuclear polyhedosis virus from Enproctis pseudoconspersa were detected by coomassie brilliant blue staining. The results revealed Ep NPV Occulusion body protein contains a single polypetide with a M · W. of 27×10 3 d and 17 EpNPV—Virion proteins. Ammino—silver staining is 50 times more Sensitive than coomassie brilliant blue staining in detecting Ep NPV occulusion body protein and 6 times more Sensitive in detecting Ep NPV Virion proteins The results of ammino-silver staining showed that Ep NPV-virion contained 25 structure polypetides with molecular weigdts ranging from 8 to 115×10 3 d and there are 8 bands which were’nt be detected in coomassie brilliant blut staining could be stained by amminc-silver staining. Besides sensitive, ammino-silver staining shows bester background and rapiply. 丙烯酰胺凝胶电泳 茶毛虫核型多角体病毒 结构多肽 铵银染色和考马斯亮蓝染色比较 1987-04-01 2021-04-01 4