利用紫外线对纤维素酶产生菌黑曲霉原生质体进行诱变的研究

利用紫外线对纤维素酶产生菌黑曲霉原生质体进行诱变的研究 ULTRAVIOLET-MUTAGENESIS OF CELLULASE PRODUCER Aspergillus niger BY USING PROTOPLASTS 曹军卫 first-author 陈漱涢 用紫外线对纤维素酶产生菌黑曲霉（Aspergillus niger）097的原生质体进行诱变的研究结果表明,原生质体的存活率随着紫外线照射时间的延长而下降。从诱变处理后的原生质体再生菌落中,筛选到若干株在孢子颜色和产纤维素酶能力上都与出发菌株有较大差异的变异菌株。其中97V 3 —3除了孢子颜色由黑色转变为褐色外,其纤维素酶不同组分酶活力都有明显的提高,羧甲基纤维素水解活力比出发菌株提高了45.41%,滤纸水解活力提高了14.29%,β-葡萄糖苷酶活力提高27.72%,棉花水解活力提高28.21%。 Ultraviolet—Mutagenesis of cel lulase producer Aspergillus niger 097 by using protoplasts were reported. Protoplasts of Aspergillus niger showed the same ultraviolet killing kinetics as int act cells. After mutagenesis, several mutants were screened in regenerated colonies These mutants are different from the original strain in spore color and abitity of producing cellulase One of these mutants designated as 97V 3 —3. In which the color of spores turned into brown, and the activities of its cellulase for carboxym ethyl cellulose, filter paper, cotton and the β-gluc osid ase were increased by 45.41%, 14.29% 28.21% and 27.72% respectively. 原生质体诱变 紫外线诱变 纤维素酶 黑曲霉 中国科学院科学基金 1987-04-01 2021-04-01 4