葡萄膜炎患者外周血单核细胞表达的疾病相关细胞因子的检测

葡萄膜炎患者外周血单核细胞表达的疾病相关细胞因子的检测 Determination of the Disease Correlative Cytokines Expressed by Peripheral Blood Mononuclear Cells from Patients of Uveitis 乔治 QIAO Zhi1 1 2 3 first-author 闵磊 MIN Lei1 1 2 3 张梁海 CHANG Liang-hai2 1 2 3 武汉大学医学院 武汉大学医学院 School of Medicine,Wuhan University School of Medicine,Wuhan University 武汉医学研究所,湖北,武汉,430071 武汉医学研究所,湖北,武汉,430071 Wuhan Institute of Medical Science Wuhan Institute of Medical Science ,湖北,武汉,430014 ,湖北,武汉,430014 用ELISA测IL 12、IFN γ水平 ;用原位杂交染色法测IL 12mRNA表达 ;电泳迁移率改变试验 （EM SA）测NF AT活性 结果表明 :葡萄膜炎患者PBMCIL 12、IFN γ水平和IL 12mRNA表达以及NF AT活性都显著高于正常对照和其他眼疾病患者 （P <0 .0 1） IL 12、IFN γ水平异常增高是葡萄膜炎患者Th1/Th2 功能紊乱、Th1细胞功能亢进主要原因之一 ;而在转录水平上NF AT活性异常增高可能与患者IFN γ基因转录增强密切相关. To detect the The levels of IFN-γand IL-12 and IL-12 gene expression and the activation of NF-AT in peripheral mononuclear cells （PBMC） in patients of uveitis and investigate their relationship on uveitis Methods The levels of IFN-γand IL-12 were detected by ELISA; the IL-12mRNA was detected by situ hybridization（ISH）respectively; the activity of NF-AT was detected by electrophoretic mobility shift assay（EMSA）. Result: The levels of IFN-γ?IL-12 and IL-12 gene expression and the activation of NF-AT in PBMC from the patients of uveitis were significance higher than normal contrast and other patients of ophthalmopathy （P<0.01）.Conclusion: The abnormal expression of IL-12?IFN-γ and NF-AT, which is suggested that may contribute to the pathogenesis of uveitis .These results prompt that the imbalance of Th 1/Th 2 cell might exist in patients with uveitis. The significance higher of the regulation by NF-AT at transcription level may be associated with reinforcement transcription of IFN-γ in uveitis. 葡萄膜炎 IL-12基因 NF-AT活性 uveitis interleukin-12 gene NF-AT activity R773.9 2002-06-01 2021-04-01 6