甘蓝型油菜抗(耐)菌核病近等基因系的创建及抗病性分析

甘蓝型油菜抗(耐)菌核病近等基因系的创建及抗病性分析 The Establishment of Sclerotinia Sclerotiorum Resisant Near Isogenic Lines 甘莉 GAN Li 1 2 first-author 伍新玲 WU Xin-ling 1 2 金良 JIN Liang 1 2 丰胜求 FENG Sheng-qiu 1 2 陈翠莲 CHAN Cui-lian 1 2 汤华 TANG Hua 1 2 华中农业大学作物遗传改良国家重点实验室 华中农业大学作物遗传改良国家重点实验室 The National Key Laboratory of Crop Genetics and Improvement, Huazhong Agricultural University The National Key Laboratory of Crop Genetics and Improvement, Huazhong Agricultural University 华中农业大学作物遗传改良国家重点实验室,湖北,武汉,430070 华中农业大学作物遗传改良国家重点实验室,湖北,武汉,430070 以抗 （耐 ）菌核病显著强于中油 82 1的中R 888、中R 783、宁RS 1作供体亲本 ,中油 82 1作受体亲本和回交亲本创建了甘蓝型油菜抗菌核病的近等基因系 经离体叶片接种菌核病菌抗性鉴定 ,结果表明 3个近等基因系 （NIL）与回交亲本抗菌核病差异极显著 （P <0 .0 1） ,其中以供体亲本中R 888品系抗病性最强 ,NIL的抗病性强弱的排列顺序与抗性亲本的相一致 油菜种子带菌率的测定结果显示 （中油 82 1×中R 888）BC5的带菌率为 0 也与供体亲本的带菌率大小顺序相一致 随机选择的油菜品种 （系 ）种子中游离脯氨酸含量分析 ,结果表明 3大类型油菜的脯氨酸含量差异极显著 ,芥菜型油菜 >NIL（甘蓝型油菜 ） >白菜型油菜 >极感病甘蓝型油菜 . Zhong R-888 （Brassice napus） had the best resistance to stem rot, Zhong R-783 （B. napus） and Ning RS-1 （B.napus） had better ressitance than Zhongyou821 （B.napus）. Three BC 5, near isogenic line （NIL） of with stem rot resistance were established by using Zhongyou821 as the receptor parent, and Zhong R-888, Zhong R-783, and Ning RS-1 as the donor parents. The resistance to stem rot was significantly different between the three NIL and Zhongyou821 at theP<0.01 level of significance. The fungi on seed ratios of the NIL were similar to that of their respective donor parents. Proline content of seeds was significantly different among the NIL at theP<0.01 levels of significance, respectively. Proline content of seeds of several randomly selected varieties of mustard, B.campestris, and the three sclerotiorum resistant NIL differed at the P<0.01 level of significance. The proline content is seeds of mustard was higher then that of the three sclerotiorum resisant NIL, B. campestris and susceptible B. napus. 甘蓝型油菜 近等基因系 抗病性 菌核病 脯氨酸 带菌率 Brassica napus NIL（near-isogenic line ） resistance disease sclerotinia sclerotiorum proline fungi-on-seed ratios S435.654 国家自然科学基金 （ 30 170 6 0 0 ）;湖北省自然科学基金资助项目 （ 2 0 0 1ABD113） 2002-06-01 2021-04-01 6