金属离子和热激处理对菊芋（HelianthustuberosusL.）类金属硫蛋白基因htMT₂表达的影响

金属离子和热激处理对菊芋（HelianthustuberosusL.）类金属硫蛋白基因htMT 2 表达的影响 Effects of Metal Ions and Heat Shock on the Expression of A Metallothionein-Like GenehtMT2 inHelianthus tuberosus 常团结 CHANG Tuan-jie1 1 2 3 first-author 刘秀花 LIU Xiu-hua1 1 2 3 刘茵 LIU Yin1 1 2 3 阎永峰 YAN Yong-feng1 1 2 3 李淑萍 LI Shu-ping1 1 2 3 姚晓惠 YAO Xiao-hui1 1 2 3 朱祯 ZHU Zhen2 1 2 3 商丘师范学院生物系 商丘师范学院生物系 Department of Biology, Shangqiu Normal College Department of Biology, Shangqiu Normal College 中国科学院遗传与发育生物学研究所,河南,商丘,476000 中国科学院遗传与发育生物学研究所,河南,商丘,476000 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences Institute of Genetics and Developmental Biology, Chinese Academy of Sciences ,北京,100101 ,北京,100101 使用不同金属离子及热激对菊芋进行了处理 ,并对菊芋不同组织器官中类金属硫蛋白基因 （htMT2 ）mRNA水平的变化进行了研究 结果表明 ,htMT2在根中不表达 ,而且其表达不受金属离子的影响 Cu2 + 降低叶中htMT2的表达 ,Cu2 + 浓度与茎中htMT2mRNA水平呈负相关性 在低浓度范围内 ,Zn2 + 浓度与茎、叶中的htMT2mRNA水平呈正相关性 ,而在高浓度范围内 ,Zn2 + 浓度与htMT2mRNA水平呈负相关性 Ca2 + 对叶中htMT2表达的影响与Zn2 + 的作用相似 ,但Ca2 + 诱导茎中htMT2mRNA水平升高 热激处理对不同组织中htMT2的表达无显著影响 研究的结果表明 ,htMT2表达受金属离子影响的特征与植物MT基因一致 ,进一步证实了我们前期工作中分离到的htMT2是一个新的植物MT基因 . The expression ofhtMT2 in tissues of Helianthus tuberosus treated with metal ions and heat shock is studied by Northern blot hybridization. The results indicate thathtMT2 is not expressed and affected by mental ions in roots. The expression in leaves and stems can be reduced by Cu 2+ and induced by low concentration of Zn 2+. However, the expression ofhtMT2 can be suppressed by Zn 2+ when its concentrations are above 500 μmol/L. The effects of Ca 2+ on the expression ofhtMT2 in leaves are similar to that of Zn 2+. But Ca 2+ induces the expression ofhtMT2 in stems. The expression levels ofhtMT2 in various tissues are not significantly affected by heat shock. The results above demonstrate that the expression ofhtMT2 can be regulated by metal ions and conform thathtMT2 is a new metallothionein-like gene. 菊芋 金属硫蛋白 金属离子 基因表达 Helianthus tuberosus L. metallothionein gene metal ion gene expression Q943.2 国家“86 3”高技术计划基金 （Z 17 0 1 0 1;BH 0 2 0 1 0 1）;国家自然科学基金项目 （ 399890 0 1,39980 0 2 4,39880 0 2 3）;国家转基因植物研究与产业化开发专项基金 （ZJY B 0 1）;中国科学院特别支持费资助项目 （STZ98 3 0 8） 2002-06-01 2021-04-01 6