猪瘟病毒野强毒株持续感染细胞模型的初步研究

猪瘟病毒野强毒株持续感染细胞模型的初步研究 Basic Characteristic of the PK-15 Model Persistently Infected with a Field Heavy Strain of Virulent CSFV22 郑从义 ZHENG Cong-yi1 1 2 3 first-author 童攒 TONG Zan1 1 2 3 应松成 YING Song-cheng1 1 2 3 屈三甫 QU San-pu1 1 2 3 王在时 WANG Zai-shi2 1 2 3 武汉大学生命科学学院 武汉大学生命科学学院 College of Life Sciences,Wuhan University College of Life Sciences,Wuhan University 中国兽药监察所,湖北,武汉,430072 中国兽药监察所,湖北,武汉,430072 China Institute of Veterinary Drug Control China Institute of Veterinary Drug Control ,北京,100081 ,北京,100081 经过筛选和连续传代 ,建立了稳定的猪瘟病毒野强毒株 （CSFV2 2 ）感染猪肾传代细胞系 （PK 15 ）的持续感染细胞模型 ,获得CSFV2 2 PK15病毒持续感染的传代细胞株 用免疫荧光技术、RT PCR技术、透射电镜、流式细胞仪研究了CSFV2 2 PK15细胞株连续传代中病毒在细胞内持续感染的基本特性 结果显示传代细胞表现为病毒持续感染的基本特征 . After selection and continual subculture, a steady, persistently infected cell model of porcine kidney cell line （PK-15） infected by a field heavy strain of virulent CSFV22 is established and thereby obtaining the CSFV22-PK15 strain persistently infected by CSFV22. Furthermore, we study the fundamental characteristics of persistently infected virus in the CSFV22-PK15 strain by means of immunofluorescence assay, RT-PCR, Transmission electron microscopy and flow cytometry. The results show that CSFV22-PK15 strain has basic features of persistently infected virus. 猪瘟病毒野强毒株 持续感染 细胞模型 细胞周期 field hearg svrain of virulent CSFV persistent infection cell model cell cycle S852.65 国家重点基础研究发展规划项目“973”（G19990 1190 4） 2002-06-01 2021-04-01 6