芽孢杆菌的异源转化

芽孢杆菌的异源转化 STUDIES ON THE HETEROLOGOUS TRANSFORMATION IN THE GENUS BACILLUS 范秀容 Fan Xiurong Lin Qinghua Fu Xiaoqin Zheng Congyi Lian Yeguang first-author 林清华 付晓琴 郑从义 连业光 <正> 遗传转化是一种比较能定向地培育优良菌种的方法,近年来国内已开始利喟这一方法进行育种（1）,但由于在大量的菌株中只有个别菌株能作为受体（2）,为了克服受体的此种局限性,节省工作量,当要求选育的菌种在种内尚未建立受体菌时,就需要利用已经建立的转化受体,进行异源转化;同时,在目前有些实验室条件较差以及用以证明转化体的DNA酶和RNA酶供应不足的情况下,转化实验所检出的转化体如何得到进一步证明,也是需要解决的问题;苏云金芽孢杆菌是国内生产较多和应用较广的杀虫剂,而此菌的遗传转化国内外均未见有报导。基于以上三个原因,本实验选用了苏云金杆菌与枯草杆菌168受体菌进行了异源转化,并试用血清学凝集反应对转化体进行鉴定。 Bacillus subtilis 168 was transformed with deoxyribonucleic acid isolated from B. thuringiensis D 57 and B. thuringiensis 7216. Streptomycin resistance was selected as a genetic marker. It has been shown that the genetic markers can be transferred to competent recipient cultures. The frequency of transformation was found to be 3×10 -5 . Serological agglutination tests were used to demonstrate the transformants. There were no cross reactions between the recipient strains and the donor strains. All of the transformants agglutinated in the antiserum against recipient, and most of them could net agglutinate in the antiserum against two donors. Hence the transformants could be distinguished from the donors. Of all the trans formants, four strains could agglutinate in the antiserums against two donors besides in the antiserum against recipient. Therefore the transformants could be distinquished from the streptomycin resistant mutant of the recipient. So we think the agglutination tests can be used as a method to help demonstrate the transformants in experiment of heterologous transformation. 1980-03-01 2021-04-01 3