蛋白质分子在硅片表面成膜的初步研究

蛋白质分子在硅片表面成膜的初步研究 STUDIES ON BrNDING OF PROTEIN MOLECULES ONTO SILICON WAFER SURFACES TO FORM THIN FILMS 杨天权 first-author 曹军卫 邱万川 费镇明 陈淑涓 研究了细菌视紫质、伴刀豆球蛋白、人血清白蛋白等几类蛋白质分子在硅片表面成膜的方法。藉椭偏术测量膜层厚度及扫描电子显微术观察膜层形貌的结果表明:物理吸附成膜不牢固!化学偶联法及亲和配体结合法可以得到附着较为牢固、厚度达3.9-16.2nm的蛋白质分子薄膜。采用化学偶联或/和亲和配体结合法制备蛋白质分子叠累膜,有希望得到宏观连通的膜层。 The methods for binding of protein molecules such as bacteriorhodopsin, cocanavaline A, human serum albumin etc. onto the silicon wafer surface were studied and compared by means of ellipsometry to estimate the thick-ness of established protein film and of scanning electron microscopy to re-veal the topography of the film. It was found that the film made by chemical coupling and affinity ligands binding methods fastened firmly on the silicon surfaces. The thickness of protein film by chemical coupling and affinity lig-and binding ranged from 3.9 to 16.2nm 蛋白质分子膜 硅片表面蛋白质膜 细菌视紫质 椭偏术测定 Protein molecules film Silicon surface binding of protein film Bacteriorhodopsin Ellipsometry 1988-03-01 2021-04-01 3