猪红细胞的铜锌超氧化物歧化酶纯化和性质的研究

猪红细胞的铜锌超氧化物歧化酶纯化和性质的研究 THE PURIFICATION AND PROPERTIES OF COPPER/ZINC SUPEROXIDE DISMUTASE FROM PORCINE ERYTHROCYTES 邹国林 Zou Guolin 1 first-author 钟晓凌 Zhong Xiaoling 1 王东梅 Wang Dongmei 1 曹新文 Cao Xinwen 1 朱汝璠 Zhu Rufan 1 湖北工学院食品工程系 进修教师 湖北工学院食品工程系 进修教师 Department of Biology, Wuhan University Department of Biology, Wuhan University 从猪红细胞中分离纯化到CuZn—SOD。纯化的酶经disc电泳显示为一条蛋白带,酶比活力为每毫克蛋白18000单位。该酶在紫外与可见光区的最大吸收分别为267nm和665nm。经SDS-PAGE测得酶亚基分子量约为16200道尔顿。根据计算,酶亚基由大约151个氨基陵残基组成,不含色氨酸。用DNS法测得N-末端氨基酸是封闭的。等电聚焦电泳得到6条酶蛋白带,其pH值分别为5.70,6.05,6.10,6.30,6.45和6.80. Copper/zinc superoxide dismutase from porcine erythrocytes has been isolated and purified. The purified enzyme was found to be homogeneous as judged by polyacrylamide gel disc e!ectrophoresis Its specific activity is 18000 units per mg protein It exhibits one absorption maximum in the ultra-violet at 267 nm and] another in the visible at 665 nm. The enzyme subunit molecular weight is about 16200 daltons as estimated by SDS-polyacrYlamide gel electrophoresis, and the subunit consists of ab’ut 151 amino acid residues by calculation, in which tryptophan residue is absent. The enzyme contains ablocked N-terminal amino acid as assayed by dansyl chloride. Six bands ofthe enzyme were seen in isoelectric’focusing, and these focused at pH 5.70, 6.05, 6.10, 6.30, 6.45 and 6.80. 猪红细胞 铜锌超氧化物歧化酶 纯化 性质 purification properties CuZn—SOD porcine erythrocytes 1988-01-01 2021-04-01 1