乙型肝炎病毒ayw亚型基因文库的构建

乙型肝炎病毒ayw亚型基因文库的构建 CONSTRUCTION OF GENE BANK FOR HEPATILIS B VIRUS ayw SUBTYPE 齐义鹏 Qi Yipeng Huang Youngxui Wei Zhongyi Lin Shengqin 1 2 first-author 黄永秀 1 2 魏中一 1 2 林胜清 1 2 武汉大学病毒学系 武汉大学病毒学系 Department of Virology, Wuhan University Department of Virology, Wuhan University 湖北医学院附一医院 湖北农学院进修教师 湖北医学院附一医院 湖北农学院进修教师 将乙型肝炎病毒（HBV）ayw亚型基因组DNA克隆在质粒pBR322的EeoRI位点,组成重组DNA分子,转化到E.coli HB101细胞中,获得转化子。经电泳、dot blot和Southem blot杂交证实,阳性重组体含有HBV DNA,组成了HBV基因文库。在E.coli细胞中,插进质粒中的HBV DNA能扩增并表达HBV抗原。 By using recombination of Hepatilis B virus （HBV） ayw subtype DNA with plasmid pBR322 on EcoRI site and then transformation into E. coli HB101, 466 transformant colonies have been observed on antibiotic-LB plates. Screen-ing by agarose gel. dot blot and Southern blot, it is indicated that HBV DNV has already been inserted into the vector for positive recombinants We have demonstrated the HBV DNV inserted into the plasmid is able to replicate and express efficiently three antigens of HBV in E. coli cells 乙型肝炎病毒 重组 基因文库 分子杂交 表达 hepatilis B virus recombination gene bank molecular hybridization gene expression 国家自然科学基金(61672102) 1989-02-01 2021-04-01 2