大麦叶鞘诱导愈伤组织的细胞学动态的研究

大麦叶鞘诱导愈伤组织的细胞学动态的研究 STUDIES ON CYTOLOGICAL CHANGES IN CALLUS TISSUES INDUCED FORM LEAF SHEATH OF BARLEY 利容千 first-author 刘立华 曾子申 本文以大麦叶鞘离体培养诱导愈伤组织过程中,观察细胞形态学的动态变化,发现培养的叶鞘组织有三种变化情况:一部分接种后,细胞组织逐渐萎缩、解体;另一部分维持生活状态,变化比较缓慢,保持到一定时期形成愈伤组织或慢慢解体;第三种情况形成正常的愈伤组织,呈一种松散絮状透明组织团块。愈伤组织多数起源于幼叶鞘的外层,即下表皮以内维管束之间的第二、三层薄壁细胞,部分发生于维管束韧皮部的薄壁细胞,也有的发生于外植体切口的一端或两端,间也有发生于叶鞘边缘地方,它们回复分裂机能逐步形成愈伤组织。接种前维管束鞘及其周围几层细胞有淀粉粒分布,接种后随着细胞的起动、脱分化、愈伤组织的形成,淀粉粒逐步解体消失,同时对于愈伤组织的起源以及三种变化情况进行了初步讨论。 Young leaf sheath explants of barley were cultured on N 6 medium supplemented with 2, 4-D （2mg/l） for induction of callus formation. Histocytological changes during callus formation were studied. After 3-5 days culture most of the callus tissues originated from the parenchyma beneath the lower epidermis and between the vascular bundles. Afterwards the callus tissues grew through the lower epidermis and formed calli. In some cases the parenchyma between phloem and lower epidermis is also capable of callus formation. Before culture starch grains were distributed in the bundle-sheath cells. But with the proceeding of callus formation they were disintegrated. the origin and cytological changes of callus tissues were discussed. 1981-04-01 2021-04-01 4