不同pH对血红蛋白与过氧化氢复合物中间体的影响

不同pH对血红蛋白与过氧化氢复合物中间体的影响 The Influence on the Compound Ⅱ of Hemoglobin with Hydrogen Peroxide by Various pH Buffer 李海成 LI Hai-cheng 1 2 first-author 李得嘉 LI De-jia 1 2 邹国林 ZOU Guo-lin 1 2 武汉大学生命科学学院 武汉大学生命科学学院 College of Life Sciences, Wuhan University College of Life Sciences, Wuhan University 武汉大学生命科学学院,湖北,武汉,430072 武汉大学生命科学学院,湖北,武汉,430072 以血红蛋白模拟过氧化物酶,在不同pH溶液中,测定并比较了催化反应中间体-Ⅱ的ks,t1/2和催化活性等.结果表明,中间体-Ⅱ的ks,t1/2和催化活性均与缓冲液的pH密切相关,其稳定性在pH5.0时最小,而此时的催化反应速度最高. With Hb as mimetic peroxidase in various pH buffer it was studied that the ks and t1/2 of the compound Ⅱwere associated with the pH of buffer, and the half-life period of the compound Ⅱ was the shortest at pH5. 0 where the catalytic reaction rate of the whole was the highest. 血红蛋白 模拟酶 动力学 hemoglobin mimetic enzyme dynamics Q591.2 国家自然科学基金资助项目（39770200） 2002-02-01 2021-04-01 2