温度对水稻谷氨酰胺合成酶和NADH-谷氨酸合酶表达的影响

温度对水稻谷氨酰胺合成酶和NADH-谷氨酸合酶表达的影响 Effect of Temperature on Expression of Glutamine Synthetase and NADH-Glutamate Synthase in Rice Plants 陆彬彬 LU Bin-bin 1 2 3 first-author 周 卫 ZHOU Wei 1 2 3 张 吉 ZHANG Ji 1 2 3 潘 磊 PAN Lei 1 2 3 林清华 LIN Qing-hua 1 2 3 张楚富 ZHANG Chu-fu 1 2 3 武汉大学植物发育生物学教育部重点实验室 武汉大学植物发育生物学教育部重点实验室 Key Laboratory of MOE for Plant Developmental Biology, Wuhan University Key Laboratory of MOE for Plant Developmental Biology, Wuhan University 武汉大学植物发育生物学教育部重点实验室,湖北,武汉,430072 武汉大学植物发育生物学教育部重点实验室,湖北,武汉,430072 湖 湖 测定了不同温度（32℃和23℃）培养下水稻幼苗根和叶的谷氨酰胺合成酶（GS）同工酶以及依赖于NADH的谷氨酸合酶（NADH-GOGAT）的活性变化.结果表明,温度对水稻根和叶的GS活性具有相反的作用.23℃下生长的水稻根的GS活性明显高于32℃下生长的活性,而23℃下生长的叶的GS活性显著低于生长在32℃下的叶的活性.Native-PAGE和活性染色以及蛋白质印迹表明,根和叶的GS活性变化主要是由于GSrb和GS2活性及其相应蛋白质水平变化所致.23℃下生长的水稻根的NADH-GOGAT活性显著高于32℃下生长的活性,而叶的活性则基本保持恒定. We compared the activity of Glutamine Synthetase （GS） and NADH-Glutamate Synthase （NADH-GOGAT） from roots and leaves of rice plants cultured under different temperature C32℃ and 23℃) , respectively. The results showed that both GS and GOGAT activities of roots from rice grew under 23℃ were obviously higher than those of grew under 32℃ , but GS activity of leaves decreased a lot, whde GOGAT activity only decreased a little. Native-PAGE combined with activity staining and immunoblotting revealed that changes of GS activity in the roots and leaves may be mainly contributed to changes of GSrb and GS2. 谷氨酰胺合成酶 谷氨酸合酶 同工酶 水稻 温度 glutamine synthetase glutamate synthase isozyme rice temperature Q946.5 国家自然科学基金（39870527）;国际合作基金资助项目 2002-02-01 2021-04-01 2