大肠杆菌HB101感受态细胞的显微观察与分析

大肠杆菌HB101感受态细胞的显微观察与分析 Study on the Competent Cells of Escherichia coli HB101 by Transmission Electron Microscope 谢志雄 XIE Zhi-xiong1 1 2 first-author 陈向东 CHEN Xiang-dong1 1 2 李文化 LI Wen-hua1 1 2 沈 萍 PANG Dai-wen2 1 2 庞代文 SHEN Ping1 1 2 武汉大学生命科学学院 武汉大学生命科学学院 College of Life Sciences, Wuhan University College of Life Sciences, Wuhan University 武汉大学化学与分子科学学院 湖北 武汉430072武汉大学化学与分子科学学院湖北,武汉,430072 武汉大学化学与分子科学学院 湖北 武汉430072武汉大学化学与分子科学学院湖北,武汉,430072 College of Chemistry and Molecular Sciences, Wuhan University College of Chemistry and Molecular Sciences, Wuhan University 对数生长前期的大肠杆菌在生理盐水中用CaCl2可以直接诱导建立感受态并完成转化过程.利用H-8100透射电子显微镜观察用CaCl2直接在生理盐水中诱导建立感受态的大肠杆菌细胞,发现感受态细胞整体电子密度趋于均匀,但存在有电子密度较高颗粒,表明大肠杆菌感受态建立过程中,细胞整体对电子通透性增强,可能存在涉及细胞膜通透性的复合物重新形成相关的胞内物质代谢及其在细胞内定位的受控改变,暗示大肠杆菌可能具有建立自然感受态的能力. In saline, CaCl2 could induce the competence development of Escherichia coli cells at pro-log phase directly. Using transmission electron microscope （H-8100）, we have investigated the competent cells of E. coli, which were induced by CaCl2 in saline immediately. The electron micrographs indicated the electron density within competent cells became uniform, but some high electron density granules e-merged along the margins of the cells. The electron permeability of the E. coli cells enhanced during competence development, this might be coincident with the metabolism alterations and recomposition of the material, which correlated to the osmolability of membrane. These suggested the competence development of E. coli be most probably internally regulated. 透射电子显微镜 大肠杆菌 感受态细胞 transmission electron microscope Escherichia coli competent cells Q939 国家自然科学基金资助（30070408,30070010）;武汉市晨光计划项目（20015005051）;武汉大学自然科技创新基金资助 2002-02-01 2021-04-01 2