粗毛栓菌漆酶的分离纯化及部分性质研究

粗毛栓菌漆酶的分离纯化及部分性质研究 Induction, Isolation and Analysis of an Extracellular Laccase from Trametes gallic Fr. 朱 陶 ZHU Tao 1 2 first-author 赵永芳 ZHAO Yong-fang 1 2 武汉大学生命科学学院 武汉大学生命科学学院 College of Life Sciences, Wuhan University College of Life Sciences, Wuhan University 武汉大学生命科学学院,湖北,武汉,430072 武汉大学生命科学学院,湖北,武汉,430072 将一株自筛的粗毛栓菌（Trametes gallic Fr）接种在含有麦草粉的培养基中可获得较高产量的漆酶,此酶可以吸附到DEAE-纤维素上而被直接从发酵液中分离出来.采用DEAE-纤维素柱层析,用含有0.1-0.9mol/L NaCl的磷酸缓冲液（pH4.5）进行梯度洗脱,可以获得较为纯化的漆酶,经研究,该菌株所产漆酶,以邻联甲苯胺为底物,最适pH值为4.0,最适反应温度40℃,Km为0.105 mmol/L. Methods are described for the analysis, production and isolation of laccase produced by a strain of Trametes gallic Fr. Using a natural medium supplemented with wheatgrass as inducer,high titer of laccase was obtained. The enzyme was isolated directerly from fermentation medium by adsorbing to di-ethylaminoethyl cellulose（DEAE-C）. It was quantitatively recovered from the resin by elution with 50mM phosphate buffer（pH4. 5）. The optimal pH optimal temperature of the laccase were 4. 0 and 40℃ respectively. Michaelis constant of it was 105 μmol/L. 漆酶 粗毛栓菌 最适温度 最适pH Laccase Trametes gallic Fr temperature optima pH optima Q55 2002-02-01 2021-04-01 2