球形改性壳聚糖的制备及吸附牛血清蛋白性能的研究

球形改性壳聚糖的制备及吸附牛血清蛋白性能的研究 Adsorption of BSA on Modified Chitosan Microsphere Resin 施晓文 SHI Xiao-wen 1 2 first-author 肖 玲 XIAO Ling 1 2 杜予民 DU Yu-min 1 2 覃彩芹 QIN Cai-qin 1 2 武汉大学资源与环境科学学院 武汉大学资源与环境科学学院 College of Resource and Environment Sciences, Wuhan University College of Resource and Environment Sciences, Wuhan University 武汉大学资源与环境科学学院,湖北,武汉,430072 武汉大学资源与环境科学学院,湖北,武汉,430072 采用反相悬浮交联法制备了壳聚糖多孔微球树脂,讨论了交联剂环氧氯丙烷浓度、致孔剂PEG2000浓度对成球性能的影响.红外光谱、光学显微镜和扫描电镜的结果表明,树脂为蜂窝状多孔微球,色泽好,粒度均匀,阴离子交换容量为3-4mmol/g,不溶于水、酸、碱.从吸附牛血清蛋白（BSA）的实验发现,交联剂浓度对吸附有很大影响,交联浓度为0.04 mol·L-1时得到的微球树脂对牛血清蛋白有较好的吸附,其饱和吸附容量达到 116mg/g. In this study, chitosan microsphere was prepared by phase-inversion method successfully. The effects of the concentration of the crosslinker and the porogen on properties of the microsphere have been discussed. The structure of the microsphere was characterized by scanning electron microscopy （SEM） and Fourier transform infrared （FT-IR）. The influence of different crosslinker concentration on the BSA adsorption was investigated. Results indicate that the microsphere prepared here has high porosity, large content of amine groups, and is insoluble in both acidic and basic solutions. It has high adsorption rate to BSA. Adsorption capacity of the resin for BSA was 116 mg/g. 壳聚糖 多孔微球树脂 吸附 chitosan microsphere adsorption O636.1 国家自然科学基金资助项目（29977014） 2002-02-01 2021-04-01 2