应用同工酶分析技术对小麦提型和斯型不育系的初步研究^*

应用同工酶分析技术对小麦提型和斯型不育系的初步研究 * A PRELIMINARY INVESTIGATION ON THE CYTOPLASMIC MALE STERILE LINES OF TR TIMOPHEEVI AND TR SPELTA BY MEANS OF ISOZYMIC ANALYSIS 徐乃瑜 Xu Naiyu Wang Xulian first-author 王许莲 三氯氢硅或烷基二氯硅烷在氯铂酸催化下与m-或p-卤代烯丙苯加成,得到十一种新型γ-卤苯基丙基三氯硅烷和γ-卤苯基丙基烷基二氯硅烷。产率一般为60～70%,个别达到80%。元素分析、红外光谱和氢硅化反应的定位规则表明它们具有预期的结构。这些单体与正硅酸乙酯共水解和聚合,得到十种新型的含有γ-卤苯基的共聚物。 An investigation made on the esterase isozymic patterns indicated that the number of isozymic bands and the type of zymograms of 545-spelta, Ja panese-spelta, common wheat anhui 11 were similar to each other, in contrast with them, those of timopheevi revealed distinct differences. The number of isozymic bands and type of zymograms of the （spelta） anhui 11 cytoplasmicsterile line were similar to those of its maintainer （B-line）, while those of the （timopheevi） anhui 11 cytoplasmic-sterile line showed some difference, but they differed greatly from those of timopheevi. An investigation made on the peroxidase isozymic patterns also revealed that the number of isozymic bands and the type of zymograms of all materials tested showed close similarities except those of timopheevi, but the differences of peroxidase zymograms between timopheevi and other materiale tested were not so obvious as those of esterase zymograms. Consequently, the analysis of esterase isozymic patterns was more helpful to the identification of the species-speificity of wheat, and the evidences as mentioned above indicated that the esterase and peroxidase isozymes of wheat were mainly coded by the nucleus genes, the exogenous cytoplasm did not exercise much effect on both isozymic patterns. 1981-03-01 2021-04-01 3