柠檬醛对黄曲霉质膜物理损伤机制的研究

柠檬醛对黄曲霉质膜物理损伤机制的研究 Study on Physical Mechanism of Citral Damage the Aspergillus Flavus’s Membrane 罗 曼 LUO Man1 1 2 3 first-author 蒋立科 JIANG Li-ke2 1 2 3 邹国林 ZOU Guo-Iin1 1 2 3 安徽农业大学生物工程系 安徽农业大学生物工程系 College of Life Sciences, Wuhan University College of Life Sciences, Wuhan University 武汉大学生命科学学院 安徽 合肥23003武汉大学生命科学学院湖北,武汉,430072 武汉大学生命科学学院 安徽 合肥23003武汉大学生命科学学院湖北,武汉,430072 Department of Biological Engineering, Anhui Agricultural University Department of Biological Engineering, Anhui Agricultural University ,安徽,合肥,23003 ,安徽,合肥,23003 通过反射干涉对比显微技术和快速图像分析技术,捕获细胞质膜外光晕变化和细胞周边边缘激发变化的信息,并采用闪烁本征模式分解测定柠檬醛作用于黄曲霉质膜前后各模式（m=0,1,2）所对应的平均平方振幅<δn2m>、剪切弹性μ和弹性弯曲模量Kc,与理论模式光谱相比,研究柠檬醛损伤质膜的物理机制.结果表明,m=1的平动模式主导了光晕波谱,其平均平方振幅呈双指数衰变,该模式几乎与剪切弹性无关,即μ=0;弹性弯曲模量Kc的最小和最大值分别为9.45×10-18J和5.43×10-17J,与柠檬醛的浓度成正相关性.揭示该醛对黄曲霉质膜的损伤,除改变膜的选择通透性和电导率外,同时使质膜平动模式的平均平方振幅呈双指数衰变,弹性弯曲模量Kc增加,从而降低膜伸缩的弹性. The change information of flicker out membrane and the edge excitations simultaneously at four orthogonal was seized to fast image processing with a resolution of 5nm combining phase contrast microscopy. The mean square amplitude <δn2m> shear elasticity μ and bending modulus Kc were measured by flicker eigenmode decomposition, before and after AFC damaged by citral Comparing these with the spectrum of theoretical pattern, the physical mechanism of citral damaging membrane of A. flavus was studied. The results displayed that the translation pattern （when its azimuthal mode number equal to one, m=1） occupied a dominant position, μ the mean square amplitude was in bi-exponential decay. There was hardly relation between the pattern and shear elasticity-i. e m=1<μ = 0>. The minimum and the maximum of Kc were respectively 9. 45 ×10-18 J and 5. 34 × 10-17 J. There existed positive relativity between Kc and the concentration of citral. The results showed that when A. flavus cell membrance was damaged by citral fell into; besides its selective permeability and electrical conductivity were affected its <δN2m=1> was in bi-exponential decay and its Kc gradually, resulting in membrane’s elasticity falling. 柠檬醛 黄曲霉质膜 反射干涉对比显微镜 本征模式分解 快速图像分析技术 Citral membrane of Aspergillus flavus reflection interference contrast microscopy eigenmode decomposition fast image analysis technology R313 国家档案局资助项目（903-保-02） 2002-02-01 2021-04-01 2