紫红链霉菌2917磷脂酶A₂基因的克隆及序列分析

紫红链霉菌2917磷脂酶A 2 基因的克隆及序列分析 Cloning and Sequencing of Phospholipase A 2 Gene from Streptomyces violaceoruber 2917 李维琳 LI Wei\|lin 1 first-author 华中农业大学生命科学技术学院,湖北,武汉,430072 华中农业大学生命科学技术学院,湖北,武汉,430072 College of Life Science and Technology,Huazhong Agricultural University College of Life Science and Technology,Huazhong Agricultural University 用探针从紫红链霉菌 2 917基因组文库中克隆出磷脂酶A2 基因 ,经双向测序 ,确定了其基因的全序列 ,并由此推导出其氨基酸序列 将此序列与其他来源的磷脂酶A2 基因序列进行了比较研究 ,结果显示 ,与天蓝色链霉菌A3（2 ）中磷脂酶A2 的同源性为 98% ;与蛇毒的磷脂酶A2 的同源性小于 10 % ,但有类似的质子传递系统、Ca2 + 结合环以及疏水通道等结构 这个基因的克隆为磷脂酶A2 结构与功能的研究提供更多的信息 ,也为利用重组微生物生产磷脂酶A2 打下了基础 The gene of Phospholipase A 2 （PLA 2 ） was cloned from Streptomyces violaceoruber 2917 genomic DNA library using PCR produce as probe. The whole sequence of the cloned gene was determined by bidirectional sequencing, and the amino acid sequence was deduced. Comparing the gene sequence with that from S. coelicolor A3（2） shows that they have a 98% homologous. Though the homology of PLA 2 gene is less than 10% between S.violaceoruber 2917 and snake venom, they have similar proton transfering system, Ca 2+ binding ring as well as hydrophobic channels. The cloning of this gene provides more information for the studying of PLA 2 structure and function, and also provides basis for the production of PLA 2 with the recombinant Streptomyces strains. 紫红链霉菌2917 磷脂酶A2 序列分析 Streptomyces violaceoruber 2917 phospholipase A 2 sequence analysis Q78;Q556 农业部农业微生物重点实验室开放课题资助项目 2002-04-01 2021-04-01 4