MA₇₈₂腹水型细胞某些表面特性的研究—在外源凝集素作用下的凝集力

MA 782 腹水型细胞某些表面特性的研究—在外源凝集素作用下的凝集力 SOME SURFACE CHARACTERISTICS OF MOUSE MAMMARY ADENOCARCINOMA(MA 782 )ASCITES CELLS——AGGLUTINABILITY INDUCED BY THE LECTIN 方世国 Fang Shiguo and Liu Yiming first-author 柳益明 外源凝集素作为一种分子探针,对癌细胞或病毒转化细胞表面特性的研究具有重要意义。已查明615小鼠可移植性乳腺腺癌(MA 782 )腹水型细胞中有病毒颗粒存在。本文对MA 782 腹水型细胞在外源凝集素作用下的凝集特性进行了研究。用分光光度法定量测定细胞的凝集动态。该细胞在PHA作用下产生显著的凝集作用,比红细胞的凝集强得多,ConA也引起了癌细胞的凝集。低温（4℃）抑制了PHA诱发的细胞凝集,在24℃下细胞明显的凝集,37℃下凝集更显著,反应速度也加快。低温抑制的细胞凝集是可逆的。固定剂戊二醛和甲醛均抑制PHA诱发的细胞凝集。本文讨论了癌细胞的凝集特性与膜的流动性问题。 Agglutinability of MA 782 ascites cells induced by the lectin was inves tigated. cell agglutination was assayed spectrophometrically. The MA 782 ascites cells were clearly agglutinated by the PHA and ConA. The PHA-induced agglutination was inhibited by low temperatures （4℃）, and this was reversed by warming of cells. Cells were clearly agglutinated at 24℃ and the agglutination was increased markedly at 37℃. The PHA-induced agglutination was inhibited by glutaraldehyde and formaldehyde. The relations between the agglutinability of tumour cells and membrane fluidity are discussed. 1982-02-01 2021-04-01 2