白鲢精巢鱼精蛋白mRNA的分离,纯化及活性测定

白鲢精巢鱼精蛋白mRNA的分离,纯化及活性测定 ISOLATION PURIFICATION AND ACTIVITY DETERMINATION OF POLY（A） + PROTAMINE mRNA FROM SILVER CARP TESTIS 曾治义 Zeng Zhiyi and Yi Jianhua first-author 易健华 本文采用Gedamu.Iatrou和Dixon等人的方法、从白鲢精巢分离、纯化Poly（A） + 鱼精蛋白mRNA.所得样品经紫外光谱测定具有典型核酸特征、以聚丙烯酰胺凝胶电泳分析呈现一区带、其分子量大小以它的迁移率同tRNA（4S）和5sRNA比较、估计接近6s.通过麦胚无细胞体系活性测定、发现C 14 —精氨酸与C 14 —异亮氨酸的掺入合成多肽。用聚丙烯酰胺——脲素凝胶电泳鉴定,证明合成的多肽产物是鱼精蛋白。 In our experiments, we used the method described by Gedamu, Iatrou and Dixon to isolate and purify the Poly（A） + protamine mRNA from Silver Carp testis. Obtained sample has the typical character of nucleic acid when detected by the U. V. absorption spectrum. Analysis on the polyacrylamide gel electrophoresis shows that the mRNA has a single band, and its molecular weight as estimated by its mobility in comparison with the markers of tRNA（4s） and 5sRNA is about 6s. The determination of its activity in the wheat germ cell-free system reveals that both C 14 -arginine and C 14 -isoleucine are incorporated into synthetic polypeptide. Identification of the poly peptide on the polyacrylamide-area gel electrophoresis proved that this synthetic polypeptide is exactly protamine. 1982-02-01 2021-04-01 2