新城疫病毒HB92株M基因的克隆和序列分析

新城疫病毒HB92株M基因的克隆和序列分析 Cloning and Sequene Analysis of the Matrix Protein Gene of Newcastle Disease Virus HB92 Strain 陈玉栋 CHEN Yu-dong 1 1 2 3 4 first-author 潘兹书 PAN Zi-shu 1 1 2 3 4 邵华斌 SHAO Hua-bin 2 1 2 3 4 杨峻 YANG Jun 2 1 2 3 4 张楚瑜 ZHANG Chu-yu 1 1 2 3 4 武汉大学生命科学院 武汉大学生命科学院 College of Life Sciences, Wuhan University College of Life Sciences, Wuhan University 湖北省农业科学院畜牧兽医研究所 湖北省农业科学院畜牧兽医研究所 Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Science Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Science 武汉大学生命科学院,湖北,武汉,430072 武汉大学生命科学院,湖北,武汉,430072 ,湖北,武汉,430209 ,湖北,武汉,430209 采用RT PCR方法扩增了新城疫病毒 （NDV）无毒耐热株 （HB92株 ）M基因 ,将其克隆于 pGEM T载体 ,并进行了序列测定和分析 .结果显示 ,测定的M基因长 12 2 3个核苷酸 ,包含一个 10 95个核苷酸的开放阅读框（ORF） ,编码 36 4个氨基酸 .与已报道的 10株NDVM基因比较 ,核苷酸同源性为 88.4 %～ 95 .8% ,氨基酸同源性为 89.8%～ 95 .3% ,HB92株与V4株M基因核苷酸的同源性最高 （达 95 .8% ） .NDVM蛋白分子中有 9对碱性氨基酸 ,在多肽的第 117～ 12 0位有一个含 4个氨基酸CKKS的特征性结构 .这些结果为揭示NDVHB92株的分子生物学背景 ,了解NDV的分子进化和遗传变异机理 ,进而开发利用HB92株奠定了基础 ,也为将NDV另列为副粘病毒亚科的一个新属提供了理论支持 M gene of Newcastle disease virus （NDV） HB92 strain, which was amplified by RT-PCR and cloned to pGEM-T vector, has been sequenced and analyzed. The complete nucleotide sequence of the M gene is 1 223 long. The major open reading frame （ORF） of 1 095 nt contains a coding region of 364 amino acid residues. A comparison of the nucleotide sequences of M gene of the HB92 strain with other 10 strains of NDV is 88.4% to 95.8% identity, and it is 89.8% to 95.3% identity as to amino acid. The homology of nucleotide is very high between HB92 and V4. There are 9 pairs of basic amino acids, 5 of which are distributing at C-terminal of M protein, in addition, there is also a unique tetrapeptide CKKS at 117 to 120 resides. These results have shown the molecule biology background of NDV HB92 strain and provide a groundwork through which we can understand the mechanism of molecule evolution of NDV and it’s genetic and variable mechanism and further utilize NDV HB92. This data is also consistent with the very recent taxonomy approved by the ICTV executive committee, which assign NDV into the new genus Avulavirus. 新城疫病毒 基质蛋白基因 序列分析 逆转录多聚酶链式反应 newcastle disease virus matrix protein gene sequence analysis RT-PCR S852.65 湖北省自然科学基金资助项目 （2 0 0 2AB14 4） 2003-04-01 2021-04-01 4