棉铃虫核型多角体病毒朝鲜分离株BamHI-Ⅰ片段的核苷酸序列分析

棉铃虫核型多角体病毒朝鲜分离株BamHI-Ⅰ片段的核苷酸序列分析 Sequence Analgsis of the BamHI-Ⅰ Gene Fragment of Korean Isolate of Helicoverpa Armigera of the Single Nucleocapsid Nucleopolyhedrovirus 赵光日 ZHAO Guang-ri 1 1 2 3 4 first-author 张珈敏 ZHANG Jia-min 1 1 2 3 4 胡远扬 HU Yuan-yang 1 1 2 3 4 武汉大学生命科学学院 武汉大学生命科学学院 College of Life Sciences, Wuhan University College of Life Sciences, Wuhan University 武汉大学生命科学学院,湖北,武汉,430072 武汉大学生命科学学院,湖北,武汉,430072 Pyongyang Agricultural University,Pyongyang, The Democratic People’s Republic of Korea Pyongyang Agricultural University,Pyongyang, The Democratic People’s Republic of Korea 平壤农业大学 平壤农业大学 ,湖北,武汉,430072 ,湖北,武汉,430072 对在朝鲜首次分离得到的棉铃虫核型多角体病毒朝鲜株 （HaSNPV KO）基因组BamHI Ⅰ片段的全序列进行了测序与分析 .该片段全长 1.895kb ,包括 p2 6基因 ,p10基因及 p74基因的 3′末端序列 .p2 6基因位于p10基因的上游 .排列方向与p10基因相同 ,该基因的编码区大小为 80 3bp ,编码 2 6 7个氨基酸 .p10基因的编码区大小为 2 6 1bp ,编码 87个氨基酸 .p74基因的排列方向与 p10和p2 6基因相反 .棉铃虫核型多角体病毒朝鲜株与棉铃虫核型多角体病毒中国株 （HaSNPV G4株 ）的p2 6基因序列的同源性为 99.8% ,两个分离株的 p10基因序列完全相同 ,而 p74基因 3′末端序列的结果有 99.4 %的同源性 .朝鲜株与美洲株 （HzSNPV）的 p2 6和 p10基因序列的同源性分别为 98.8% ,98.7% ,而 p74基因 3′末端序列有 97.9%的同源性 After carrying on measuring and analysis the sequence of the BamHI-Ⅰ fragment of the genomic organization of the Korean isolate HaSNPV firstly, we find it has 1 895 bp.The fragment contained p26 gene, p10 gene, 3′end of p74 gene.The transcriptional direction of the p26 gene is identical with that of the p10 gene. The ORF encoding p26 was 803 nucleotide long and encoding a putative 267 amino acid, and the ORF encoding p10 was 261 nucleotide long and encoding a putative 87 amino acid. But it is opposite to that of the p74 gene.The identity of p26 and p10 gene of Korean isolate of Helicoverpa armigera are respectively 99.8 and 100 percent with HaSNPV（G4）, but it is 99.4 percent with the 3’end of p74 gene.The identity of p26 and p10 gene are respectively 98.8 and 98.7 percent in the HaSNPV-KO and HzSNPV, but it is 97.9 percent with the 3′end of p74 gene. 棉铃虫核型多角体病毒朝鲜株 p26基因 p10基因 p74基因 序列分析 HaSNPV-KO p26 gene p10 gene p74 gene sequence analysis S476.1 2003-04-01 2021-04-01 4