玉米和类玉米杂交后代异染色质纽的遗传稳定性分析

玉米和类玉米杂交后代异染色质纽的遗传稳定性分析 Genetic Stability Analysis of Heterochromatic Knob in Progeny of Distant Hybrid Between Maize and Teosint 韩永华 HAN Yong-hua 1 1 2 3 4 5 6 first-author 郭乐群 GUO Le-qun 3 1 2 3 4 5 6 李冬郁 LI Dong-yu 4 1 2 3 4 5 6 谷明光 GU Ming-guang 3 1 2 3 4 5 6 宋运淳 SONG Yun-chun 1 1 2 3 4 5 6 武汉大学生命科学学院/植物发育生物学教育部重点实验室 武汉大学生命科学学院/植物发育生物学教育部重点实验室 College of Life Sciences,Wuhan University/Key Laboratory of MOE for Plant Developmental Biology College of Life Sciences,Wuhan University/Key Laboratory of MOE for Plant Developmental Biology 中国科学院遗传研究所 中国科学院遗传研究所 College of Life Sciences, Guangxi Normal University College of Life Sciences, Guangxi Normal University 广西畜牧研究所 广西畜牧研究所 Institute of Genetics, The Chinese Academy of Sciences Institute of Genetics, The Chinese Academy of Sciences 武汉大学生命科学学院/植物发育生物学教育部重点实验室,湖北,武汉,430072 武汉大学生命科学学院/植物发育生物学教育部重点实验室,湖北,武汉,430072 Guangxi Institute of Animal Sciences Guangxi Institute of Animal Sciences 广西师范大学生命科学学院 广西师范大学生命科学学院 ,广西,南宁,530001 ,广西,南宁,530001 利用玉米的近缘亲属摩擦禾的基因组DNA ,对玉米自交系 330、二倍体多年生类玉米及其远缘杂交孤雌生殖后代异源种质纯系 5 4 0的染色体进行比较基因组原位杂交 ,发现上述 3个供试种染色体上的异染色质纽因杂交信号极强而能被清楚地显示 .与亲代相比 ,5 4 0中出现异染色质纽数目减少的现象 .并讨论了造成这种现象的可能原因 Molecular banding undertaken with comparative genomic in situ hybridization （cGISH） is a simple approach which generates chromosome characteristic signals in different species at regions with conserved repeated sequences.The knobs gave strong hybridization signals and easily be identified when mitotic chromosomes from maize inbred 330, DP and alloplasmic pure line 540 which derived from the parthenocarpic progenies of a backcrossed distant hybrid between 330 DP were hybridized with total genomic DNA from Tripsacum dactyloides. The results indicated the number of knobs reduced on the chromosomes of pure line 540 compared to its parents and possible reason of this change was discussed. 玉米 二倍体多年生类玉米 摩擦禾 异染色质纽 比较基因组原位杂交 分子显带 Zea mays ssp. mays Zea diploperennis Tripsacum dactyloides heterochromatic knob comparative genomic in situ hybridization （cGISH） molecular banding S513.03 国家自然科学基金资助项目 （ 3 0 0 70 3 76） 2003-04-01 2021-04-01 4