油桐尺蠖核型多角体病毒及其基因组某些理化性质研究

油桐尺蠖核型多角体病毒及其基因组某些理化性质研究 CHARACTERIZATION OF NUCLEAR POLYHEDROSIS VIRUS DNA FROM BUZURA SUPPRESSARIA 陈海雷 Chen Hailei Liu Niencui 1 first-author 刘年翠 1 武汉大学病毒及分子生物学系 武汉大学病毒及分子生物学系 Department of Virology and Molecular Biology, Wuhan University Department of Virology and Molecular Biology, Wuhan University 油桐尺蠼核型多角体病毒（BsNPV）的多角体大小为1～1.5μm×0.8～1.6μm。病毒粒子长度为0.31～0.8μm。直径为0.05～0.06μm。在0.1×SSC溶液中测得其基因组DNA T m 值为76.5℃,（G+C）%为55.14%,电镜法测得其DNA分子平均长度为41.3μm,计算出对应分子量为81.36×10 6 daltons。用限制性内切酶混合酶解后琼脂塘凝胶电泳,测出DNA积加分子量为79.36×10 6 daltons（120.23kb）。 The range in size of Buzura Suppressaria NPV occlusion body was 1～1.5μm×1～1.5μm with polyhedron shape, and virion was rod-shaped, 0.31—0.34μm in length and 0.05-0.06μm in diameter. Tm of viral genome in 0.1×SSC solution was 76.5℃. The （G+C）% content determined by Tm was 55.14%. The average length of BsNPV DNA determined by electron microscopy was 41.3μm, corresponding to a molecular weight of 81.36×10 8 daltons. The viral genome was examined with restriction endonuclearase, single and double digestion DNA using BamHI/Bgl Ⅱ KpnI/XhoI etc. and analyzed in 0.7～1.0% agarose gel electrophresis, the result of which showed a total moleclar weight of DNA genome of 79.35×10 6 daltons （120.23kb）. The length of DNA fragment was calculated by Southern’s formula in computer. 油桐尺蠼NPV DNA Tm值 (G+C)％含量及分子量 限制性内切酶酶解 国家科学基金 1989-03-01 2021-04-01 3