嗜碱性芽孢杆菌（Bacillus sp）NT-39及其所产碱性淀粉酶的初步研究

嗜碱性芽孢杆菌（Bacillus sp）NT-39及其所产碱性淀粉酶的初步研究 PRELIMINARY STUDIES ON CHARACTERISTIC OF ALKALOPHILIC BACTERIA BACILLUS SP.NT-39 AND ITS ALKALINE AMYLASE 田小群 Tian Xiaoqun 1 first-author 武汉大学生物系 武汉大学生物系 Department of Biology,Wuhan University Department of Biology,Wuhan University 从我国内蒙古碱性土壤中分离得到的嗜碱芽孢杆菌（Baciflus sp.）NT—39是一产碱性淀粉酶的菌株。最适生长 pH 为10。革兰氏染色阴性。能利用多种糖、醇类物质作为碳源生长。利用麦芽糖作碳源,产酶活力最高,其次是糊精和可溶性淀粉。利用有机氮源时,产酶活力比利用无机氮源时高。发酵液粗酶制品的作用最适条件为 pH 9,50℃.Ca 2+ 对酶的热稳定性有保护作用。酶在 pH6～11范围内比较稳定。Ca 2+ 、Mn 2+ 对酶有激活作用,Ag + 、Hg 2+ ,Zn 2+ 、Pb 2+ 、Al 3+ ,Fe 3+ 有抑制作用。 Alkalophilic bacteria Bacillus sp.NT-39 was isolated from soil in china. The biological characters of this strain are as follow:It has an optimum- growth pH at 10,and no growth at pH 7.Utilizes a lot of carbon compounds as carbon source for growth,ferments glucose,but don’t grow on the medium containing 2% NaCl.When maltose was utilized as carbon source amylase activity was the highest.Using organic nitrogen as nitrogen source amylase activity was higher than inoranie nitrogen.The optimum action condition of crude enzyme preparation was pH9,50℃.Enzyme stable at pH 6～11.Ca 2 protected the enzyme from heat.Ca 2 and Mn 2 were activa tots of this enzyme and Ag,Hg 2 ,Zn 2 ,Pb 2 ,Al 3 and Fe 3 were inhibitors. 嗜碱性细菌 碱性淀粉酶 生物学特性 产酶条件 国家自然科学基金资助的课题 1990-02-01 2021-04-01 2