嗜碱细菌碱性果胶酶的研究——Ⅰ.菌种筛选、产酶条件和酶作用条件的研究

嗜碱细菌碱性果胶酶的研究——Ⅰ.菌种筛选、产酶条件和酶作用条件的研究 STUDIES ON ALKALINE PECTINASE FROM ALKALOPHILIC BACTERIA Ⅰ.Selection of Strains and Studies on the Culture Conditions for Enzyme Producing and Enzyme Properties 黄诗笺 Huang Shijian Lu Zhenzu Chen Shuyun 1 first-author 卢振祖 1 陈漱涢 1 武汉大学生物系 武汉大学生物系 Departement of Biology,Wuhan University Departement of Biology,Wuhan University 从碱性土壤中分离得到的97株嗜碱细菌,从中筛选到15个产碱性果胶酶活力较高的菌株,摇瓶发酵酶活力范围1467～2500u·ml -1 .其中被鉴定为专性嗜碱芽孢杆菌（Bacillussp.）的 HL-6菌株在 pH8～11.5培养基中生长良好,在 pH7以下不生长。该菌产碱性果胶酶的最适培养条件为:以2%果胶和2%乳糖为复合碳源,培养基起始 pH10～10.5,温度37℃,摇瓶培养60h 达产酶高峰。0.5%甘氨酸提高酶活平均达34%。在上述条件下,芽孢杆菌LH-6菌株摇瓶发酵的粗酶液以果胶为底物,酶作用的最适 pH 为11.5,最适温度为60℃。 Fifteen strains producing high activity of alkaline pectinase were selected from the ninety-six alkalophilic bacteria isolated from alkaline soil.The range of the enzyme activity produced by above strains with shalking was 1467-2500 u·ml -1 . Bacillus sp.LH 6 was determined as a stable obligate alkalophilic and alkline pectinase producing bacteria.This microorganism had good growth in pH 8-11.5 media,and no growth occurred below pH7.The culture conditions for the enzyme production was investigated.The organism was grown in the medium containing complex carbon source（2%（w/v）pectin and 2%（w/v） lactose）at an initial pH of 9.7 and 37℃,and the highest enzyme activity was obtained after 60 h cultivation with shalking.Glycine（0.2—1% w/v）was evidently effective to stimulate the production of the enzyme,and the average rate of increase in the enzyme activity produced by the organism was reached 34% at 0.5%（w/v）.The investigation on some properties of the crude enzyme solution produced by Bacillus sp.LH 6 under above culure conditions indicated when the pectin was used as the substrate of the enzymes, the optimum pH and temperature for the enzyme activity was 11.5 and 60℃. 嗜碱芽孢杆菌 菌种筛选 碱性果胶酶 alkalophilie Bacillus sp. selection of the strains alkaline pectinase 国家自然科学基金资助课题 1990-01-01 2021-04-01 1